WB
H M R Mk
Endogenous
58
Rabbit
#P53041
5536
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human PP5. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Protein phosphatase 5 (PP5) is a member of the serine/threonine phosphatase family that also includes PP1, PP2A and PP2B. PP5 uniquely contains an amino-terminal regulatory domain with three tetratricopeptide repeat (TPR) motifs and a carboxy-terminal catalytic domain (1-3). Through the TPR domain, PP5 interacts with a number of proteins and has been reported to be involved in diverse signal transduction pathways, including glucocorticoid receptor signaling, p53-mediated growth arrest, oxidative stress-mediated apoptosis and the response to ionizing radiation-induced DNA damage (4-8).
- Chen, M.X. and Cohen, P.T. (1997) FEBS Lett. 400, 136-140.
- Andreeva, A.V. and Kutuzov, M.A. (1999) Cell. Signal. 11, 555-562.
- Chinkers, M. Trends Endocrinol. Metab. 12, 28-32.
- Zuo, Z. et al. (1998) J. Biol. Chem. 273, 12250-12258.
- Morita, K. et al. (2001) EMBO J. 20, 6028-6036.
- Silverstein, A.M. et al. (1997) J. Biol. Chem. 272, 16224-16230.
- Ali, A. et al. (2004) Genes Dev. 18, 249-254.
- Wechsler, T. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 1247-1252.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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