Revision 1
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, IP

REACTIVITY:

H M Mk

SENSITIVITY:

Endogenous

MW (kDa):

110

Source/Isotype:

Rabbit IgG

UniProt ID:

#P21127

Entrez-Gene Id:

984

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

PITSLRE/CDK11 (D88B3) Rabbit mAb recognizes endogenous levels of total PITSLRE/CDK11 protein. This antibody will detect the full-length (CDK11p110) and is predicted to detect the alternate transcript (CDK11p58) of PITSLRE/CDK11. The antibody is predicted to detect both PITSLREA/CDK11B and PITSLREB/CDK11A.

Species Reactivity:

Human, Mouse, Monkey

Species predicted to react based on 100% sequence homology

Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met734 of human PITSLRE/CDK11 protein.

Background

PITSLRE, alternatively known as cell division kinase 11 (CDK11), is the result of duplication of the CDK11 gene (1). CDK11A and CDK11B encode nearly identical serine/threonine protein kinases, PITSLREB and PITSLREA respectively, both belonging to the p34CDC2 family of protein kinases (2). Full-length PITSLRE/CDK11 (commonly referred to as CDK11p110) is expressed ubiquitously throughout the cell cycle whereas a smaller, alternate transcript (CDK11p58), the result of internal ribosomal entry, is expressed only during the G2/M transition where it promotes centrosome maturation and spindle formation (3-5). During induction of apoptosis by Fas or TNF, or anoikis, PITSLRE/CDK11 is cleaved by caspases to generate p110C, an approximately 46 kDa protein that contains the catalytically active kinase domain of PITSLRE/CDK11 that interacts with and inhibits p21-activated kinase (PAK1) activity (6-8). Full length PITSLRE/CDK11 (CDK11p110) appears to participate in pre-mRNA splicing events. This is demonstrated by the observation that CDK11p110 interacts with numerous splicing factors including RNPS1, 9G8/SRSF7 and cyclin L and that CDK11p110 can phosphorylate and inhibit the splicing activity of 9G8/SRSF7 (9-11).

  1. Gururajan, R. et al. (1998) Genome Res 8, 929-39.
  2. Xiang, J. et al. (1994) J Biol Chem 269, 15786-94.
  3. Cornelis, S. et al. (2000) Mol Cell 5, 597-605.
  4. Petretti, C. et al. (2006) EMBO Rep 7, 418-24.
  5. Wilker, E.W. et al. (2007) Nature 446, 329-32.
  6. Chen, S. et al. (2003) J Biol Chem 278, 20029-36.
  7. Lahti, J.M. et al. (1995) Mol Cell Biol 15, 1-11.
  8. Ariza, M.E. et al. (1999) J Biol Chem 274, 28505-13.
  9. Hu, D. et al. (2003) J Biol Chem 278, 8623-9.
  10. Loyer, P. et al. (1998) J Cell Sci 111 ( Pt 11), 1495-506.
  11. Dickinson, L.A. et al. (2002) J Biol Chem 277, 25465-73.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。

专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品, (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Revision 1
#5524

PITSLRE/CDK11 (D88B3) Rabbit mAb

Western Blotting Image 1: PITSLRE/CDK11 (D88B3) Rabbit mAb Expand Image
使用 PITSLRE/CDK11 (D88B3) Rabbit mAb 对不同细胞系的提取物进行蛋白质印迹分析。
No image available