Phospho-Jak1 (Tyr1034/1035)/Jak2 (Tyr1007/1008) (E9Y7V) Mouse mAb (#66245) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, IP

REACTIVITY:

H M

SENSITIVITY:

Endogenous

MW (kDa):

125, 130

Source/Isotype:

Mouse IgG2a

UniProt ID:

#O60674

Entrez-Gene Id:

3717

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-Jak1 (Tyr1034/1035)/Jak2 (Tyr1007/1008) (E9Y7V) Mouse mAb recognizes endogenous levels of Jak2 protein only when phosphorylated at Tyr1007 and Tyr1008 and endogenous levels of Jak1 when phosphorylated at Tyr1034 and Tyr1035. This antibody may cross-react with phospho-Jak3 and phospho-Tyk2.

Species Reactivity:

Human, Mouse

Species predicted to react based on 100% sequence homology

Rat, Monkey, Bovine, Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1007/Tyr1008 of human Jak2 protein.

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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