WB, IP
H
Endogenous
50
Rabbit
#P11912
973
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Bovine, Dog
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr210 of human CD79A protein. Antibodies are purified by peptide affinity chromatography.
Background
Antigen receptors found on the surface of B cells contain a heterodimeric signaling component composed of CD79A and CD79B, also known as Ig α and Ig β, respectively (1,2). Presence of this receptor complex is essential for B cell development and function (3). Together these two proteins and the associated B cell receptor (BCR) initiate intracellular signaling following antigen binding (4,5). An immunoreceptor tyrosine-based activation motif (ITAM) found in the CD79A intracellular region appears to be important for its function (6). Antigen binding precedes formation of the CD79A and CD79B heterodimer and subsequent activation of receptor associated kinases (7). Research has shown that CD79A is a marker for B-lineage lymphoblastic leukemia (8). Additionally, investigators have found that mutations in the CD79A (MB1) gene are associated with abnormally low levels of functional B cell receptors in some cases of chronic B cell lymphocytic leukemia (9).
Tyrosine 210 is an evolutionarily conserved non-ITAM tyrosine residue that lies within the cytoplasmic domain of CD79A/Ig-alpha. Research studies have shown that ligation of the B cell receptor (BCR) induces phosphorylation of tyrosine 210, which facilitates both the recruitment of BLNK to the BCR signaling complex and the activation of downstream Syk-dependent signaling (10,11). Collectively, these molecular events drive B cell activation and proliferation (12).
- van Noesel, C.J. et al. (1991) J Immunol 146, 3881-8.
- Minegishi, Y. et al. (1999) J Clin Invest 104, 1115-21.
- Yu, L.M. and Chang, T.W. (1992) J Immunol 148, 633-7.
- Storch, B. et al. (2007) Eur J Immunol 37, 252-60.
- Mason, D.Y. et al. (1995) Blood 86, 1453-9.
- Luisiri, P. et al. (1996) J Biol Chem 271, 5158-63.
- Pike, K.A. et al. (2004) J Immunol 172, 2210-8.
- Astsaturov, I.A. et al. (1996) Leukemia 10, 769-73.
- Vuillier, F. et al. (2005) Blood 105, 2933-40.
- Engels, N. et al. (2001) Eur J Immunol 31, 2126-34.
- Kabak, S. et al. (2002) Mol Cell Biol 22, 2524-35.
- Patterson, H.C. et al. (2006) Immunity 25, 55-65.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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