Revision 1

#44463

Store at -20C

Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit mAb
CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H

Sensitivity:
Transfected Only

MW (kDa):
110-130, 125-140, 180-200

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q06418, #P30530, #Q12866

Entrez-Gene Id:
7301, 558, 10461

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit mAb detects immunoprecipitated or transfected levels of Axl/Mer/Tyro3 protein phosphorylated at Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681). The antibody may cross-react with other phosphorylated tyrosine proteins.

Species predicted to react based on 100% sequence homology

Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-peptides corresponding to residues surrounding Tyr698/Tyr749 of human Axl/Mer protein.

Background

Axl, Mer and Tyro3 are three members of the TAM family receptor tyrosine kinase that share a common NCAM (neural adhesion molecule)-related extracellular domain and a conserved intracellular tyrosine kinase domain. These receptors bind common homologous vitamin K dependent protein GAS6 and protein S to activate downstream signaling pathways (1). TAM family receptors are involved in the development of immune, nervous, vascular and reproductive systems, autoimmune disease, cancer drug resistance and tumor immunity response (2-5). Axl (Tyr698), Axl (Tyr702), Mer Tyr(749) and Tyro3 (Tyr681) are conserved autophosphorylation sites located in the activation loop of the respective tyrosine kinase domains. Phosphorylation at these sites is required for full kinase activation of each of the corresponding receptors (6,7).

Background References

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

#44463

Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit mAb

Cell Signaling Technology Logo
Western blot analysis of extracts from NCI-H1299 and SK-MEL-5 cells, untreated (-) or treated with GAS6 (400 ng/ml) and H2O2 (4 mM) for 10 min, using Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit mAb (upper) and Axl (C89E7) Rabbit mAb #8661 (lower 1), Mer (D21F11) XP® Rabbit mAb #4319 (lower 2), and Tyro3 (D38C6) Rabbit mAb #5585 (lower 3).
Western Blotting Image 1: Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit mAb
Immunoprecipitation of Phospho-Axl (Tyr698) protein from NCI-H1299 cells treated with GAS6 (400 ng/ml) and H2O2 (4 mM) for 10 min. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, lane 3 is Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) Rabbit mAb, and lane 4 is Axl (C89E7) Rabbit mAb #8661. Western blot analysis was performed using Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) Rabbit mAb (upper) and Axl (C89E7) Rabbit mAb #8661 (lower). Anti-rabbit IgG, HRP-linked Antibody #7074 was used as secondary antibody.
Immunoprecipitation Image 1: Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit mAb
Immunoprecipitation of Phospho-Mer (Tyr749) protein from SK-MEL-5 cells treated with GAS6 (400 ng/ml) and H2O2 (4 mM) for 10 min. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, lane 3 is Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) Rabbit mAb, and lane 4 is Mer(D21F11) XP® Rabbit mAb #4319. Western blot analysis was performed using Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) Rabbit mAb (upper) and Mer (D21F11) XP® Rabbit mAb #4319 (lower). Anti-rabbit IgG, HRP-linked Antibody #7074 was used as secondary antibody.
Immunoprecipitation Image 2: Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

#44463

Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit mAb

Cell Signaling Technology Logo
Immunoprecipitation of Phospho-Tyro3 (Tyr681) protein from SK-MEL-5 cells treated with GAS6 (400 ng/ml) and H2O2 (4 mM) for 10 min. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, lane 3 is Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) Rabbit mAb, and lane 4 is Tyro 3 (D38C6) Rabbit mAb #5585. Western blot analysis was performed using Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) Rabbit mAb (upper) and Tyro3 (D38C6) Rabbit mAb #5585 (lower). Anti-rabbit IgG, HRP-linked Antibody #7074 was used as secondary antibody.
Immunoprecipitation Image 3: Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.