Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (#23706) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, IP, IF-F

REACTIVITY:

H M R

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Rabbit IgG

UniProt ID:

#P37840

Entrez-Gene Id:

6622

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunofluorescence (Frozen)	1:400 - 1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier-free (BSA and azide free) version of this product see product #87281.

Specificity / Sensitivity

Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb recognizes endogenous levels of α-Synuclein protein only when phosphorylated at Ser129. Specificity of nuclear staining is unclear but consistent with previous publications (4).

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser129 of human α-Synuclein protein.

Background

α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-Synuclein gene are linked to Parkinson's disease (1).

Various research studies have shown that phosphorylation of α-Synuclein at Ser129 is a highly toxic event causing degeneration of dopaminergic neurons associated with Parkinson's disease. This is proposed to occur through increased misfolding, aggregation, and accumulation of α-Synuclein phosphorylated at this site (2). GSK-3β is one of several kinases that has been reported to phosphorylate α-Synuclein at Ser129 (3).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation IF-F: Immunofluorescence (Frozen)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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