WB, IP, IHC-P, FC-FP
H M R
Endogenous
75
Rabbit IgG
#Q86UX7
83706
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg648 of human Kindlin-3 protein.
Background
The kindlin family of focal adhesion proteins is involved in multiple biological processes, including integrin signaling, adhesion, migration, angiogenesis, differentiation, and mitotic spindle formation (1,2). Kindlin family members 1, 2, and 3 (FERM1, FERM2, and URP2) are differentially expressed in tissues. Kindlin-1 is primarily expressed in epithelial cells, kindlin-2 is ubiquitously expressed, and kindlin-3 expression is restricted to the hematopoietic system (3).
Kindlin-3 (FERMT3 or URP2) is a member of the kindlin family of focal adhesion proteins, which plays an essential role in integrin activation and in various immune functions (3,4). Kindlin-3 is mainly expressed in the hematopoietic system (5). Kindlin-3, together with talin, binds to the cytoplasmic tail of β-integrin and activates integrin-mediated signaling cascades (6-8). Research studies identified mutations of Kindlin-3 that cause a rare hereditary human disease, leukocyte-adhesion deficiency (LAD) type III (9,10).
- Ye, F. and Petrich, B.G. (2011) Curr Opin Hematol 18, 356-60.
- Meves, A. et al. (2009) Trends Cell Biol 19, 504-13.
- Karaköse, E. et al. (2010) J Cell Sci 123, 2353-6.
- Fagerholm, S.C. et al. (2014) Am J Clin Exp Immunol 3, 37-42.
- Pasini, E.M. et al. (2006) Blood 108, 791-801.
- Tadokoro, S. et al. (2003) Science 302, 103-6.
- Wegener, K.L. et al. (2007) Cell 128, 171-82.
- Moser, M. et al. (2009) Science 324, 895-9.
- Malinin, N.L. et al. (2009) Nat Med 15, 313-8.
- Svensson, L. et al. (2009) Nat Med 15, 306-12.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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