IMP2 (D4R2F) Rabbit mAb (#14672) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, FC-FP

REACTIVITY:

H M Mk

SENSITIVITY:

Endogenous

MW (kDa):

66, 62

Source/Isotype:

Rabbit IgG

UniProt ID:

#Q9Y6M1

Entrez-Gene Id:

10644

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Flow Cytometry (Fixed/Permeabilized)	1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

IMP2 (D4R2F) Rabbit mAb recognizes endogenous levels of total IMP2 protein. This antibody recognizes both the 66 kDa isoform 1 and the 62 kDa isoform 2. This antibody may cross-react with an unidentified protein of 40 kDa.

Species Reactivity:

Human, Mouse, Monkey

Species predicted to react based on 100% sequence homology

Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human IMP2 protein.

Background

Insulin-like growth factor-II mRNA-binding proteins (IMPs) belong to a family of zipcode-binding proteins (1,2). Three members of this family, IMP1, IMP2, and IMP3, have been identified (1,2). They contain two RNA recognition motifs, four K homology domains, and were found to function in mRNA localization, turnover, and translation control (1,2). Research studies have implicated these proteins in a variety of physiological and pathological processes, such as growth and development (3), testicular neoplasia (4), and melanocytic neoplasia (5).
A well-characterized polymorphism in the corresponding IGF2BP2 gene is associated with susceptibility to type 2 diabetes in specific ethnic populations (6).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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