Endogenous
16
Rabbit
#P49789
2272
Product Information
Storage
Specificity / Sensitivity
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the amino terminus of human FHIT. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The fragile histidine triad (FHIT) tumor suppressor gene is frequently altered in human tumors (1). Adenovirus-mediated FHIT gene transfer has been shown to arrest tumor cell growth and induce apoptosis (2,3), and FHIT-/- knockout mice have a higher incidence of spontaneous tumor formation (4,5). FHIT physically associates with ubiquitin-conjugating enzyme 9 (6), and Src has been reported to phosphorylate Tyr114 of FHIT in vitro and in vivo (7). FHIT is a diadenosine P1,P3-triphosphate hydrolase (8), but a His96Asn mutation, which reduces FHIT enzymatic activity by three-fold, does not reduce FHIT tumor suppressor activity (8,9).
- Ohta, M. et al. (1996) Cell 84, 587-597.
- Ji, L. et al. (1999) Cancer Res. 59, 3333-3339.
- Ishii, H. et al. (2001) Cancer Res. 61, 1578-1584.
- Baffa, R. et al. (1998) Cancer Res. 58, 4708-4714.
- Campiglio, M. et al. (1999) Cancer Res. 59, 3866-3869.
- Shi, Y. et al. (2000) Biochem. J. 352 , 443-448.
- Pekarsky, Y. et al. (2004) Proc. Natl. Acad. Sci. U S A 101, 3775-3779.
- Barnes, L. D. et al. (1996) Biochemistry 35, 11529-11535.
- Siprashvili, Z. et al. (1997) Proc. Natl. Acad. Sci. U S A 94, 13771-13776.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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