WB
H
Transfected Only
180
Rabbit IgG
#Q8NHM5
84678
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the human FBXL10 protein.
Background
F-box and leucine-rich repeat protein 10 (FBXL10), also known as lysine-specific demethylase 2B (KDM2B) and JmjC domain-containing histone demethylation protein 1B (JHDM1B), is a lysine-specific histone demethylase protein that demethylates Lys4 and Lys36 of histone H3 (1,2). FBXL10 contains a zinc finger-CxxC5 DNA binding domain that binds to un-methylated CpG dinucleotides and has been shown to function as part of a non-canonical polycomb repressor complex (PRC1) that is associated with repression of developmentally regulated genes (3-5). In embryonic stem cells, FBXL10 is critical for targeting PRC1 to CpG islands and regulating gene expression during differentiation. In addition, FBXL10 is over-expressed in various cancers where it functions to induce cell proliferation and repress senescence through repression of the p15Ink4b gene locus (6-8). High expression of FBXL10 in pancreatic ductal adenocarcinoma is associated with metastasis, while high expression in acute myeloid leukemia is associated with increased proliferation and self-renewal of leukemic stem cells (6-8).
- He, J. et al. (2008) Nat Struct Mol Biol 15, 1169-75.
- Frescas, D. et al. (2007) Nature 450, 309-13.
- Boulard, M. et al. (2015) Nat Genet 47, 479-85.
- Wu, X. et al. (2013) Mol Cell 49, 1134-46.
- Farcas, A.M. et al. (2012) Elife 1, e00205.
- Tzatsos, A. et al. (2013) J Clin Invest 123, 727-39.
- Nakamura, S. et al. (2013) Mol Carcinog 52, 57-69.
- He, J. et al. (2011) Blood 117, 3869-80.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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