WB
H M R Mk
Endogenous
127
Rabbit IgG
#Q16531
1642
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Chicken, Bovine
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly832 of human DDB-1 protein.
Background
Damaged DNA-Binding Protein (DDB) consists of a 127 kDa subunit (DDB-1) and a 48 kDa subunit (DDB-2) that contribute to the formation of the UV-damaged DNA-binding protein complex (UV-DDB) (1-3). In conjunction with CUL4A and ROC-1, the UV-DDB complex forms an E3 ubiquitin ligase that recognizes a broad spectrum of DNA lesions such as cyclobutane pyrimidine dimers, 6-4 photoproducts, apurinic sites and short mismatches. The complex polyubiquitinates components of the nucleotide excision repair pathway (4-6). Loss of DDB activity has been identified in a subset of xeroderma pigmentosum complementation group E (XP-E) patients and has been linked to the deficient repair of cyclobutane pyrimidine dimers in cells derived from these patients (7-10).
DDB-1 is a relatively abundant protein that is vital for normal cell function and is evolutionarily conserved in mammals, insects, worms and plants. Unlike DDB-2, lesions in DDB-1 have yet to be indentified in XP-E patients. In association with ROC-1 and CUL4A, DDB-1 functions to recruit substrate-specific targeting subunits, generally known as DCAFs or CDWs, to CUL4-RING E3 ubiquitin-protein ligase complexes (11,12). Ubiquitination of histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage by the DDB1-DDB2-CUL4A-ROC1 E3 ubiquitin-protein ligase complex may facilitate their removal from the nucleosome in order to promote DNA repair (13-15). DDB-1, in association with other CUL4-based E3 ligase complexes, has also been found to be a regulator of mTOR signaling (16,17).
- Reardon, J.T. et al. (1993) J Biol Chem 268, 21301-8.
- Keeney, S. et al. (1993) J Biol Chem 268, 21293-300.
- Hwang, B.J. and Chu, G. (1993) Biochemistry 32, 1657-66.
- Chu, G. and Chang, E. (1990) Proc Natl Acad Sci USA 87, 3324-7.
- Hirschfeld, S. et al. (1990) Mol Cell Biol 10, 2041-8.
- Payne, A. and Chu, G. (1994) Mutat Res 310, 89-102.
- Chu, G. and Chang, E. (1988) Science 242, 564-7.
- Nichols, A.F. et al. (1996) J Biol Chem 271, 24317-20.
- Kataoka, H. and Fujiwara, Y. (1991) Biochem Biophys Res Commun 175, 1139-43.
- Keeney, S. et al. (1992) Mutat Res 273, 49-56.
- He, Y.J. et al. (2006) Genes Dev 20, 2949-54.
- Lee, J. and Zhou, P. (2007) Mol Cell 26, 775-80.
- Wang, H. et al. (2006) Mol Cell 22, 383-94.
- Kapetanaki, M.G. et al. (2006) Proc Natl Acad Sci USA 103, 2588-93.
- Guerrero-Santoro, J. et al. (2008) Cancer Res 68, 5014-22.
- Ghosh, P. et al. (2008) Cell Cycle 7, 373-81.
- Hu, J. et al. (2008) Genes Dev 22, 866-71.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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