WB
H
Endogenous
290
Rabbit
#Q9HCK8
57680
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CHD8 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
CHD8 belongs to the chromodomain helicase DNA-binding (CHD) family of ATP-dependent chromatin remodeling proteins (1). The CHD family of proteins has been shown to play an important role in regulating gene expression by utilizing the energy derived from ATP hydrolysis to alter chromatin architecture (1,2). The nine CHD family members are characterized by the presence of two tandem chromodomains in the N-terminal region and an SNF2-like ATPase domain near the central region of the protein (2-4). In addition, CHD8 contains three CR (conserved region) domains, a SANT (switching-defective protein 3, adaptor 2, nuclear receptor co-repressor, transcription factor IIB)-like domain, two BRK (brahma and kismet) domains, and a DNA-binding domain (2). The chromatin remodeling activity of CHD8 has been shown to be important for the regulation of a wide variety of genes, such as the HOX genes (5) and genes that are driven by β-catenin (6), p53 (7), estrogen receptor (8), or androgen receptor (9). CHD8 can also interact with the insulator binding protein CTCF and is required for CTCF insulator activity at multiple gene loci (10).
- Hargreaves, D.C. and Crabtree, G.R. (2011) Cell Res 21, 396-420.
- Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40.
- Delmas, V. et al. (1993) Proc Natl Acad Sci U S A 90, 2414-8.
- Woodage, T. et al. (1997) Proc Natl Acad Sci U S A 94, 11472-7.
- Yates, J.A. et al. (2010) FEBS Lett 584, 689-93.
- Thompson, B.A. et al. (2008) Mol Cell Biol 28, 3894-904.
- Nishiyama, M. et al. (2009) Nat Cell Biol 11, 172-82.
- Caldon, C.E. et al. (2009) Mol Cell Biol 29, 4623-39.
- Menon, T. et al. (2010) Mol Endocrinol 24, 1165-74.
- Ishihara, K. et al. (2006) Mol Cell 23, 733-42.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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