WB
H
Endogenous
38-40
Rabbit
#P09564
924
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile69 of human CD7 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
CD7 is a type-I transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD7 is one of the earliest surface markers to be expressed on the surface of developing T cells and its expression is maintained throughout maturation of multiple T cell subsets and NK cells (1-3). Engagement of CD7 through binding its ligand, SECTM1, has been shown to promote tyrosine phosphorylation of its cytoplasmic domain, recruitment of PI3K, and delivery of costimulatory signals for T cell activation (4-6). While CD7 is expressed on normal T cells, it is also highly expressed in a variety of T cell malignancies, which has poised it as a potential target of immunotherapy (7-9).
- Haynes, B.F. et al. (1988) J Exp Med 168, 1061-80.
- Lobach, D.F. et al. (1985) J Immunol 135, 1752-9.
- Rabinowich, H. et al. (1994) J Immunol 152, 517-26.
- Ward, S.G. et al. (1995) Eur J Immunol 25, 502-7.
- Chan, A.S. et al. (1997) J Immunol 159, 934-42.
- Lyman, S.D. et al. (2000) J Biol Chem 275, 3431-7.
- Cooper, M.L. et al. (2018) Leukemia , .
- Png, Y.T. et al. (2017) Blood Adv 1, 2348-2360.
- Gomes-Silva, D. et al. (2017) Blood 130, 285-296.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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