WB, IHC-P
H
Endogenous
100-140
Rabbit IgG
#P30203
923
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:200 |
Storage
For a carrier free (BSA and azide free) version of this product see product #36549.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CD6 protein.
Background
T cell differentiation antigen CD6 is a cell adhesion molecule expressed on immature thymocytes and mature T cells, and has also been detected on a subset of B cells and NK cells within the immune system (1-4). CD6 mediates cell-cell interactions through its binding partner CD166/ALCAM (2), and contributes to the formation and maturation of the immunological synapse (3,4). CD6 functions as a co-stimulatory receptor, promoting T cell activation and proliferation through the TCR/CD3 complex signaling cascade (3-6). Studies have shown CD6 can be glycosylated (7), hyperphosphorylated on serine and threonine residues (8), and phosphorylated on tyrosine residues (6,9), each of which can differentially affect the function and signaling of this molecule. CD6 also functions as a calcium-dependent pattern receptor that binds and aggregates Gram-positive and Gram-negative bacteria. In response to lipopolysaccharide, CD6 mediates activation of the inflammatory response and secretion of pro-inflammatory cytokines (10).
- Bowen, M.A. et al. (1997) J Immunol 158, 1149-56.
- Hassan, N.J. et al. (2004) Eur J Immunol 34, 930-40.
- Gimferrer, I. et al. (2004) J Immunol 173, 2262-70.
- Zimmerman, A.W. et al. (2006) Blood 107, 3212-20.
- Roncagalli, R. et al. (2014) Nat Immunol 15, 384-392.
- Hassan, N.J. et al. (2006) Mol Cell Biol 26, 6727-38.
- Escoda-Ferran, C. et al. (2014) FEBS Lett 588, 2805-13.
- Swack, J.A. et al. (1991) J Biol Chem 266, 7137-43.
- Wee, S. et al. (1993) J Exp Med 177, 219-23.
- Sarrias, M.R. et al. (2007) Proc Natl Acad Sci USA 104, 11724-9.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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