Revision 4

#92635

Store at -20C

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IHC-P, IF-F, IF-IC

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
29

Source/Isotype:
Rabbit IgG

UniProt ID:
#P22676

Entrez-Gene Id:
794

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:50 - 1:200
Immunofluorescence (Frozen) 1:100 - 1:400
Immunofluorescence (Immunocytochemistry) 1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #87716.

Specificity/Sensitivity

Calretinin (E7R6O) XP® Rabbit mAb recognizes endogenous levels of total Calretinin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Calretinin protein.

Background

Calretinin (29 kDa calbindin, calbindin 2) is a calcium-binding protein of the EF-hand family encoded by the CALB2 gene. It is differentially expressed from homologous family member calbindin-d28k in distinct neuronal populations of the retina, auditory system, and cerebellar granule cells (1,2), and acts as a marker for specific neuronal subsets of the subthalamic nucleus and the substantia nigra (3). Calretinin has been shown to play an important role in modulating neuronal excitability and the induction of long-term potentiation (1). Research has shown that, pathologically, Calretinin is a selective marker for epithelial mesothelioma, making it a diagnostic tool to differentiate from adenocarcinomas (4).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: Human M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。

专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品 , (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 4

Cell Signaling Technology Logo
Western blot analysis of extracts from various tissues and cell lines using Calretinin (E7R6O) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 1: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded normal human brain using Calretinin (E7R6O) XP® Rabbit mAb.
Immunohistochemistry Image 1: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded normal human colon using Calretinin (E7R6O) XP® Rabbit mAb.
Immunohistochemistry Image 2: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 4

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human smooth muscle adjacent to esophageal carcinoma using Calretinin (E7R6O) XP® Rabbit mAb.
Immunohistochemistry Image 3: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Calretinin (E7R6O) XP® Rabbit mAb.
Immunohistochemistry Image 4: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Calretinin (E7R6O) XP® Rabbit mAb.
Immunohistochemistry Image 5: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 4

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human mesothelioma using Calretinin (E7R6O) XP® Rabbit mAb.
Immunohistochemistry Image 6: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human granulosa cell tumor of the ovary using Calretinin (E7R6O) XP® Rabbit mAb.
Immunohistochemistry Image 7: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Calretinin (E7R6O) XP® Rabbit mAb.
Immunohistochemistry Image 8: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 4

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin's lymphoma using Calretinin (E7R6O) XP® Rabbit mAb.
Immunohistochemistry Image 9: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the tongue using Calretinin (E7R6O) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Images depict two fields of view of the same case.
Immunohistochemistry Image 10: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded normal mouse brain using Calretinin (E7R6O) XP® Rabbit mAb.
Immunohistochemistry Image 11: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 4

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded normal mouse colon using Calretinin (E7R6O) XP® Rabbit mAb.
Immunohistochemistry Image 12: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded MKN-45 cell pellet (left, positive) or PANC-1 cell pellet (right, negative) using Calretinin (E7R6O) XP® Rabbit mAb.
Immunohistochemistry Image 13: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Confocal immunofluorescent analysis of mouse retina at low magnification (left) and high magnification (right) using Calretinin (E7R6O) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Calbindin (D1I4Q) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #65152 (red pseudocolor). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 1: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 4

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of mouse hippocampal formation using Calretinin (E7R6O) XP® Rabbit mAb (green) and GFAP (GA5) Mouse mAb (Alexa Fluor® 647 Conjugate) #3657 (cyan pseudocolor). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Calbindin (D1I4Q) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #65152 (red pseudocolor). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 2: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Confocal immunofluorescent analysis of mouse dentate gyrus using Calretinin (E7R6O) XP® Rabbit mAb (green) and GFAP (GA5) Mouse mAb (Alexa Fluor® 647 Conjugate) #3657 (cyan pseudocolor). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Calbindin (D1I4Q) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #65152 (red pseudocolor). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 3: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Confocal immunofluorescent analysis of mouse striatum using Calretinin (E7R6O) XP® Rabbit mAb (green) and GFAP (GA5) Mouse mAb (Alexa Fluor® 647 Conjugate) #3657 (cyan pseudocolor). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Calbindin (D1I4Q) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #65152 (red pseudocolor). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 4: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 4

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of mouse cerebellum (left), colon (middle), and kidney (right) using Calretinin (E7R6O) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissues were then labeled using Calbindin (D1I4Q) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #65152 (red pseudocolor). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 5: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Confocal immunofluorescent analysis of MKN-45 cells (left, positive) or PANC-1 cells (right, negative) using Calretinin (E7R6O) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 1: Calretinin (E7R6O) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.