Revision 6

#41608Store at -20C

1 个试剂盒

(9 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
β-Amyloid (D54D2) XP® Rabbit mAb 8243 20 µl 5 kDa Rabbit IgG
β-Amyloid (D3D2N) Mouse mAb 15126 20 µl 5 kDa Mouse IgG1
NeuN (D4G4O) XP® Rabbit mAb 24307 20 µl 46-55 kDa Rabbit IgG
Synaptophysin (7H12) Mouse mAb (IF Formulated) 9020 20 µl Mouse IgG1
PSD95 (D27E11) XP® Rabbit mAb 3450 20 µl 95 kDa Rabbit IgG
Cleaved Caspase-3 (Asp175) Antibody 9661 20 µl 17, 19 kDa Rabbit 
Cleaved PARP (Asp214) (D6X6X) Rabbit mAb 94885 20 µl 89 kDa Rabbit IgG
GFAP (E6N9L) Mouse mAb 34001 20 µl 50 kDa Mouse IgG2a
HS1 (D5A9) XP® Rabbit mAb 3892 20 µl 80 kDa Rabbit IgG

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit provides an economical means of detecting proteins to confirm neuronal viability and surrounding astrocytes and microglia in mouse models by immunofluorescence.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage. The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques, a major pathological hallmark of Alzheimer’s disease (1). Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (2). Glial fibrillary acidic protein (GFAP) is the main intermediate filament in mature brain astroglial and radial glial cells and GFAP also plays an important role in modulating astroglial motility and shape (3). HS1 is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (4). Previous work identifying markers of specific brain cell types using RNA-seq has shown HS1 to be a useful and specific tool to study microglia (5). Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that occurs in presynaptic vesicles of neurons (6). Postsynaptic Density protein 95 (PSD95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein involved in the assembly and function of the postsynaptic density complex (7,8). Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, including nuclear enzyme poly (ADP-ribose) polymerase (PARP) (9). PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (10). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (11).

  1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
  2. Mullen, R.J. et al. (1992) Development 116, 201-11.
  3. Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.
  4. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
  5. Zhang, Y. et al. (2014) J Neurosci 34, 11929-47.
  6. Wiedenmann, B. et al. (1986) Proc Natl Acad Sci U S A 83, 3500-4.
  7. Cao, J. et al. (2005) J Cell Biol 168, 117-26.
  8. Chetkovich, D.M. et al. (2002) J Neurosci 22, 6415-25.
  9. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
  10. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-8.
  11. Oliver, F.J. et al. (1998) J Biol Chem 273, 33533-9.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

    限制使用

    除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。

    专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品, (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

    Revision 6
    #41608

    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit

    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 1 Expand Image
    Simple Western™ analysis of lysates (0.1 mg/mL) from mouse brain using PSD95 (D27E11) XP® Rabbit mAb #3450. 虚拟泳道式图像(左图)显示一抗稀释比例为 1:10 和 1:50 时的靶标条带(如图所示)。对应的电泳图(右图)为一抗稀释比例在 1:10(蓝线)和 1:50(绿线)时沿毛细血管内分子量的化学发光结果。在还原条件下,使用 66-440 kDa 分离模块在 ProteinSimple(BioTechne 品牌)的 Jess™ Simple Western 仪器上进行该实验。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 2 Expand Image
    对经 Etoposide #2200(25 mM;5 小时)处理的 Jurkat 提取物裂解的 caspase-3 进行免疫沉淀分析。泳道 1 为 10% input,泳道 2 为 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900,泳道 3 为 Cleaved Caspase-3 (Asp175) Antibody。使用 Cleaved Caspase-3 (Asp175) Antibody 进行蛋白质印迹法分析。Anti-rabbit IgG, HRP-linked Antibody #7074 用作二抗。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 3 Expand Image
    使用 Cleaved Caspase-3 (Asp175) Antibody #9661 对经过细胞色素 C 处理的 Jurkat 细胞的裂解物 (0.1 mg/mL) 进行 Simple Western™ 分析。虚拟泳道视图(左图)显示一抗稀释比例为 1:10 和 1:50 时的两个靶标条带(如图所示)。对应的电泳图(右图)为一抗稀释比例在 1:10(蓝线)和 1:50(绿线)时沿毛细血管内分子量的化学发光结果。在还原条件下,使用 12-230 kDa 分离模块在 ProteinSimple(BioTechne 品牌)的 Jess™ Simple Western 仪器上进行该实验。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 4 Expand Image
    使用 β-Amyloid (D3D2N) Mouse mAb,对人 Aβ-37 肽、Aβ-40 肽和 Aβ-42 肽进行蛋白质印迹分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 5 Expand Image
    使用 NeuN (D4G4O) XP® Rabbit mAb(上)和 β-Actin (D6A8) Rabbit mAb #8457(下)对小鼠脑细胞、C2C12 细胞和大鼠脑细胞提取物进行蛋白质印迹分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 6 Expand Image
    使用 GFAP (E6N9L) Mouse mAb(上图)或 β-Actin (D6A8) Rabbit mAb #8457(下图)对不同组织和细胞系的提取物进行蛋白印迹分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 7 Expand Image
    使用 PSD95 (D27E11) XP® Rabbit mAb 对人小脑和大鼠脑细胞的提取物进行蛋白质印迹分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 8 Expand Image
    使用 HS1 (D5A9) XP® Rabbit mAb 对 Baf3、32D 和小鼠脾脏的细胞提取物进行蛋白质印迹法分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 9 Expand Image
    使用 β-Amyloid (D54D2) XP® Rabbit mAb,对人 Aβ-42 肽、Aβ-40 肽、Aβ-39 肽、Aβ-38 肽和 Aβ-37 肽 (5ng) 进行蛋白质印迹分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 10 Expand Image
    使用 Synaptophysin (7H12) Mouse mAb (IF Formulated) (绿色) 与 CNPase (D83E10) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #5715 (左,红色),对小鼠脑(左图)与小鼠视网膜(右图)组织进行共聚焦免疫荧光分析。蓝色伪彩 = DRAQ5®#4084(DNA 荧光染料)。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 11 Expand Image
    使用 Cleaved PARP (Asp214) (D6X6X) Rabbit mAb(上图)或 β-Actin (D6A8) Rabbit mAb #8457(下图),对未经处理 (-) 或经 Staurosporine #9953(1 μM,3 小时)处理的血清饥饿 Neuro-2a 细胞的提取物进行蛋白质印迹法分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 12 Expand Image
    使用 Caspase-3 Antibody #9662(上)或 Cleaved Caspase-3 (Asp175) Antibody(下)对未经、经十字孢碱处理(3 小时,1 µM,体内)或经细胞色素 c 处理(1 小时,0.25 mg/ml,体外)的 HeLa、NIH/3T3 和 C6 细胞提取物进行蛋白质印迹分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 13 Expand Image
    使用 β-Amyloid (D3D2N) Mouse mAb,对石蜡包埋的人阿尔茨海默病脑进行免疫组织化学分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 14 Expand Image
    使用 NeuN (D4G4O) XP® Rabbit mAb 对石蜡包埋的小鼠小脑细胞进行免疫组织化学分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 15 Expand Image
    Immunohistochemical analysis of paraffin-embedded cynomolgus monkey brain using NeuN (D4G4O) XP® Rabbit mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 16 Expand Image
    使用 GFAP (E6N9L) Mouse mAb 作为一抗对小鼠脑细胞的提取物进行蛋白印迹分析。各种抗小鼠同型特异性抗体(上图)和 Anti-mouse IgG, HRP-linked Antibody #7076(下图)用作二抗。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 17 Expand Image
    使用 PSD95 (D27E11) XP® Rabbit mAb(红色)和 Neurofilament-L (DA2) Mouse mAb #2835(绿色),对大鼠小脑和视网膜细胞进行共聚焦免疫荧光分析。蓝色伪彩 = DRAQ5® #4084(DNA 荧光染料)。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 18 Expand Image
    使用 HS1 (D5A9) XP® Rabbit mAb 对石蜡包埋的小鼠脾脏进行免疫组织化学分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 19 Expand Image
    使用 β-Amyloid (D54D2) XP® Rabbit mAb,对所示量的人 Aβ-42 肽(左)和 Aβ-40 肽(右)进行蛋白质印迹分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 20 Expand Image
    使用 Cleaved PARP (Asp214) (D6X6X) Rabbit mAb(上图)或 β-Actin (D6A8) Rabbit mAb #8457(下图),对未经处理 (-) 或已经过 Staurosporine #9953(1 μM,6 小时;+)处理的血清饥饿 H-4-II-E 细胞的提取物进行蛋白质印迹法分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 21 Expand Image
    使用 Cleaved Caspase-3 (Asp175) Antibody 对石蜡包埋的人扁桃体细胞进行免疫组织化学分析,显示其位于凋亡细胞(低倍和高倍放大)中细胞浆和核周。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 22 Expand Image
    使用 β-Amyloid (D3D2N) Mouse mAb(绿色)和 β3-Tubulin (D71G9) XP® Rabbit mAb #5568(红色),对来自 13 月龄野生型小鼠(左)和 Tg2576 小鼠(中)的脑切片进行共聚焦免疫荧光分析。在右侧显示皮质 β-淀粉样蛋白斑的详细视图。蓝色伪彩 = DRAQ5® #4084(DNA 荧光染料)。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 23 Expand Image
    使用 NeuN (D4G4O) XP® Rabbit mAb 对石蜡包埋的小鼠结肠(肠肌丛)细胞进行免疫组织化学分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 24 Expand Image
    对小鼠脑组织提取物的 GFAP 蛋白进行免疫沉淀。泳道 1 为 10% 输入,泳道 2 为 Mouse (E5Y6Q) mAb IgG2a Isotype Control #61656,泳道 3 为 GFAP (E6N9L) Mouse mAb。使用 GFAP (E6N9L) Mouse mAb 进行蛋白印迹分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 25 Expand Image
    使用 HS1 (D5A9) XP® Rabbit mAb 对石蜡包埋的 LL2 同源肿瘤进行免疫组织化学分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 26 Expand Image
    使用 β-Amyloid (D54D2) XP® Rabbit mAb,对人 Aβ-42 肽 (1 ng) 和 AD 患者的人脑脊液 (CSF) 进行蛋白质印迹分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 27 Expand Image
    对经 Staurosporine #9953(1 μM,3 小时)处理的 Neuro-2a 细胞提取物的裂解活化 PARP (Asp214) 进行免疫沉淀。泳道 1 为 10% input,泳道 2 为 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900,泳道 3 为 Cleaved PARP (Asp214) (D6X6X) Rabbit mAb。使用 Cleaved PARP (Asp214) (D6X6X) Rabbit mAb 进行蛋白质印迹法分析。Anti-rabbit IgG, HRP-linked Antibody #7074 用作二抗。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 28 Expand Image
    使用 Cleaved caspase-3 (Asp175) antibody在 SignalSlide Cleaved Caspase-3 IHC controls #8104 对未经处理(左图)或经过依托泊苷处理(右图)的石蜡包埋的 Jurkat 细胞进行免疫组织化学分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 29 Expand Image
    使用 NeuN (D4G4O) XP® Rabbit mAb 对石蜡包埋的人多形性恶性胶质瘤细胞进行免疫组织化学分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 30 Expand Image
    对过表达突变型人 APP695 的小鼠 Tg2576 脑细胞进行共聚焦免疫荧光分析。切片用 GFAP (E6N9L) Mouse mAb(绿色,小鼠 IgG2a)、β-Amyloid (D3D2N) Mouse mAb #15126(红色,小鼠 IgG1)和 HS1 (D5A9) XP® Rabbit mAb (Rodent Specific) #3892(品红色,兔 IgG)标记。样本用 ProLong® Gold Antifade Reagent with DAPI #8961(蓝色)封片。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 31 Expand Image
    Confocal immunofluorescent analysis of fixed frozen mouse cortex from wild-type (left) or an amyloid mouse model of Alzheimer's disease (right) using HS1 (D5A9) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #35363 (red) and ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 32 Expand Image
    使用 Cleaved PARP (Asp214) (D6X6X) Rabbit mAb 对未经处理(左图,阴性)或已经过 Staurosporine #9953(右图,阳性)处理的石蜡包埋的 3T3 细胞沉淀物进行免疫组织化学分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 33 Expand Image
    使用经对照肽(左)或 Cleaved Caspase-3 (Asp175) Blocking Peptide #1050(右)预孵育的 Cleaved Caspase-3 (Asp175) Antibody 对石蜡包埋的小鼠胚胎细胞进行免疫组织化学分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 34 Expand Image
    使用 NeuN (D4G4O) XP® Rabbit mAb(绿色)对小鼠海马(左)、皮质(中)和小脑(右)细胞进行共聚焦免疫荧光分析。肌动蛋白微丝由 DyLight 554 Phalloidin #13054(红色)标记。蓝色伪彩 = DRAQ5® #4084(DNA 荧光染料)。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 35 Expand Image
    对过表达突变型人 APP695 的小鼠 Tg2576 脑细胞进行共聚焦免疫荧光分析。首先用 HS1 (D5A9) XP® Rabbit mAb(绿色)和 APP/β-Amyloid (NAB228) Mouse mAb #2450(黄色)标记切片。用 Mouse (G3A1) mAb IgG1 Isotype Control #5415 封闭游离二抗结合位点之后,切片用 GFAP (GA5) Mouse mAb (Alexa Fluor® 647 Conjugate) #3657(红色)孵育。胞核用 Hoechst 33342 #4082(蓝色)标记。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 36 Expand Image
    使用 β-Amyloid (D54D2) XP® Rabbit mAb #8243(绿色)和 GFAP (GA5) Mouse mAb #3670(红色)对阿尔茨海默病淀粉样蛋白小鼠模型的小数皮下质进行共聚焦免疫荧光分析。样本用 ProLong® Gold Antifade Reagent with DAPI #8961(蓝色)封片。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 37 Expand Image
    使用 Cleaved PARP (Asp214) (D6X6X) Rabbit mAb 对石蜡包埋的 E14 大鼠胚胎进行免疫组织化学分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 38 Expand Image
    使用 GFAP (E6N9L) Mouse mAb #34001(绿色)、DyLight 650 Phalloidin #12956(红色)和 DAPI #4083(蓝色)对 U-251 MG 细胞(左图,阳性)或 HeLa 细胞(右图,阴性)进行共聚焦免疫荧光分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 39 Expand Image
    使用 HS1 (D5A9) XP® Rabbit mAb(绿色)对 32D 细胞(左图)和 C2C12 细胞(右图)进行共聚焦免疫荧光分析。蓝色伪彩 = DRAQ5® #4084(DNA 荧光染料)。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 40 Expand Image
    使用 Cleaved PARP (Asp214) (D6X6X) Rabbit mAb 对未经处理(左图,阴性)或经 Staurosporine #9953(右图,阳性)处理的石蜡包埋的 H-4-II-E 细胞沉淀物进行免疫组织化学分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 41 Expand Image
    对未经处理(左图)或经 staurosporine #9953 处理(右图)且用 Cleaved Caspase-3 (Asp175) Antibody(绿色)标记的 HT-29 细胞进行共聚焦免疫荧光图像分析。肌动蛋白纤丝用 Alexa Fluor® 555 phalloidin #8953(红色)进行标记。蓝色伪彩 = DRAQ5®(DNA 荧光染料)。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 42 Expand Image
    Flow cytometric analysis of NIH/3T3 cells (blue, negative) and 32D clone 3 cells (green, positive) using HS1 (D5A9) XP® Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 用作二抗。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 43 Expand Image
    使用 Cleaved PARP (Asp214) (D6X6X) Rabbit mAb 对石蜡包埋的小鼠卵巢进行免疫组织化学分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 44 Expand Image
    使用与非特异性阴性对照抗体(红色)相对比的 Cleaved Caspase-3 (Asp175) Antibody 对未经(蓝色)或经依托泊苷 #2200(绿色)处理的 Jurkat 细胞进行流式细胞分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 45 Expand Image
    使用 Cleaved PARP (Asp214) (D6X6X) Rabbit mAb 对石蜡包埋的小鼠脾脏进行免疫组织化学分析。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 46 Expand Image
    使用 Cleaved PARP (Asp214) (D6X6X) Rabbit mAb(绿色)对未经处理(左图,阴性)或经 Staurosporine #9953(1 μM, 3 小时;右图,阳性)处理的 Neuro-2a 细胞进行共聚焦免疫荧光分析。肌动蛋白微丝由 DyLight 554 Phalloidin #13054(红色)标记。蓝色伪彩 = DRAQ5® #4084(DNA 荧光染料)。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 47 Expand Image
    使用 Cleaved PARP (Asp214) (D6X6X) Rabbit mAb(实线)或浓度匹配的 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900(虚线)对未经处理(蓝色)或经 Staurosporine #9953(1 μM,3 小时;绿色)处理的血清饥饿 Neuro-2a 细胞进行流式细胞分析。Anti-rabbit IgG (H+L)、F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 作为二抗。
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 48 Expand Image
    使用 Cleaved PARP (Asp214) (D6X6X) Rabbit mAb(实线)或浓度匹配的 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900(虚线)对未经处理(蓝色)或经 Staurosporine #9953(1 μM,3 小时;绿色)处理的血清饥饿 H-4-II-E 细胞进行流式细胞分析。Anti-rabbit IgG (H+L)、F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 作为二抗。