| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Beclin-1 (D40C5) Rabbit mAb | 3495 | 20 µl | 60 kDa | Rabbit IgG |
| LC3A/B (D3U4C) XP® Rabbit mAb | 12741 | 20 µl | 14, 16 kDa | Rabbit IgG |
| Atg5 (D5F5U) Rabbit mAb | 12994 | 20 µl | 55 kDa | Rabbit IgG |
| Atg12 (D88H11) Rabbit mAb | 4180 | 20 µl | 16, 55 kDa | Rabbit IgG |
| Atg16L1 (D6D5) Rabbit mAb | 8089 | 20 µl | 66, 68 kDa | Rabbit IgG |
| Atg7 (D12B11) Rabbit mAb | 8558 | 20 µl | 78 kDa | Rabbit IgG |
| Atg3 Antibody | 3415 | 20 µl | 40 kDa | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Autophagy Antibody Sampler Kit provides an economical means to investigate the molecular machinery of autophagy within the cell. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.
Storage
Background
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).
- Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
- Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
- Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88.
- Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92.
- Mizushima, N. et al. (1998) Nature 395, 395-8.
- Suzuki, K. et al. (2001) EMBO J 20, 5971-81.
- Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79.
- Shintani, T. et al. (1999) EMBO J 18, 5234-41.
Background References
Trademarks and Patents
限制使用
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