Asymmetric-Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit mAb (#3505) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, IP, IHC-P, IF-IC

REACTIVITY:

H M R Mk

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Rabbit IgG

UniProt ID:

#P11940

Entrez-Gene Id:

26986

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:25
Immunohistochemistry (Paraffin)	1:1000
Immunofluorescence (Immunocytochemistry)	1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit mAb detects endogenous levels of PABP1 only when methylated on Arg455 or Arg460.

Species Reactivity:

Human, Mouse, Rat, Monkey

Species predicted to react based on 100% sequence homology

Chicken, Horse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PABP1 in which Arg455 and Arg460 are methylated.

Background

Poly(A)-binding protein 1 (PABP1) associates with the 3' poly(A) tail of mRNA and also eIF4F (1,2). eIF4F is a complex whose functions include the recognition of the mRNA 5' cap structure (eIF4E), delivery of an RNA helicase to the 5' region (eIF4A), bridging of the mRNA and the ribosome (eIF4G), and circularization of the mRNA via interaction between eIF4G and the poly(A) binding protein (PABP). PABP1 has been shown to have multiple functions including translation initiation, mRNA stabilization, and mRNA turnover (3,4). Phosphorylation of PABP has been shown to enhance RNA binding in eukaryotes, and PABP1 has been shown to shuttle between the nucleus and cytoplasm (5,6). PABP1 is methylated on Arg455 and Arg460 by the CARM1 protein methyltransferase (7,8); however, the function of this methylation has yet to be determined.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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