SignalStain® Apoptosis (Cleaved Caspase-3) IHC Detection Kit (#12692) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

IHC-P

UniProt ID:

#P42574

Entrez-Gene Id:

836

Product Information

Storage

9579 Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb: Store at -20°C.
12960 Rabbit (DA1E) mAb IgG XP^® SignalStain^® Isotype Control: Store at -20°C.
5425 Normal Goat Serum: Store at -20°C.
8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit): Store at 4°C.
11724 SignalStain^® DAB Diluent: Store at 4°C.
11725 SignalStain^® DAB Chromogen Substrate: Store at 4°C.
8112 SignalStain^® Antibody Diluent: Store at 4°C.
9997 Tris Buffered Saline with Tween^® 20: Store at room temperature.

Specificity / Sensitivity

SignalStain^® Apoptosis (Cleaved Caspase-3) IHC Detection Kit detects endogenous levels of the activated caspase-3 large fragment (17/19 kDa) resulting from cleavage adjacent to Asp175. This antibody does not recognize full-length caspase-3 or other cleaved caspases. This kit was developed for and is recommended for immunohistochemistry only.

Source / Purification

Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp175 of human caspase-3 protein. Rabbit (DA1E) mAb IgG XP^® SignalStain^® Isotype Control is not directed against any known antigen. It functions as an isotype control for rabbit IgG monoclonal antibodies.

Product Description

SignalStain^® Apoptosis (Cleaved Caspase-3) IHC Detection Kit allows the detection of activated caspase-3 in formalin-fixed paraffin-embedded human and mouse tissue samples. Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb is detected by the polymer based, HRP-conjugated SignalStain^® Boost IHC Detection Reagent in combination with SignalStain^® DAB Diluent and Chromogen Concentrate. Also included is a concentration-matched rabbit monoclonal IgG control to verify the specificity of staining.

This combination of reagents provides a sensitive and specific means of detecting apoptotic events in tissue samples.

Background

Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStain is a registered trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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