WB
H M R
Endogenous
72
Rabbit
#P08133
309
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr645 of human annexin A6 protein. Antibodies are purified by peptide affinity chromatography.
Background
Annexin A6 (ANXA6) belongs to the highly conserved annexin family of proteins characterized by their calcium (Ca2+)-dependent binding to phospholipids (1-3). The protein is highly expressed in most tissues and is predominantly located at the plasma membrane and the endosomal compartment, where it regulates cell migration and endosome trafficking by organizing membrane domains, interacting with signaling complexes, and forming transient membrane-actin interactions upon Ca2+ stimulation (3). It also mediates intracellular cholesterol homeostasis (3,4). Annexin A6 is frequently upregulated in extracellular vesicles (EVs) of the tumor microenvironment after chemotherapy or tyrosine kinase inhibitor treatment. These EVs have been shown to promote resistance and metastasis in multiple cancer types, such as lung cancer, breast cancer, and pancreatic duct adenocarcinoma (5-7). Annexin A6, in these EVs, enhances the stability of membrane-associated RTKs, such as EGFR, and promotes cancer cell motility, stemness, and autophagy (2,7-9).
- Gerke, V. and Moss, S.E. (2002) Physiol Rev 82, 331-71.
- Korolkova, O.Y. et al. (2020) Cells 9, 1855. doi: 10.3390/cells9081855.
- Grewal, T. et al. (2017) Cell Adh Migr 11, 288-304.
- Enrich, C. et al. (2011) Biochim Biophys Acta 1813, 935-47.
- Keklikoglou, I. et al. (2019) Nat Cell Biol 21, 190-202.
- Leca, J. et al. (2016) J Clin Invest 126, 4140-4156.
- Li, T. et al. (2021) Cell Death Dis 12, 684.
- Sakwe, A.M. et al. (2011) Exp Cell Res 317, 823-37.
- Guo, Z. et al. (2021) Front Cell Dev Biol 9, 718721.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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