| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb | 2478 | 20 µl | 230 kDa | Rabbit IgG |
| Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb | 4060 | 20 µl | 60 kDa | Rabbit IgG |
| Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb | 6943 | 20 µl | 60 kDa | Rabbit IgG |
| Phospho-FAK (Tyr397) (D20B1) Rabbit mAb | 8556 | 20 µl | 125 kDa | Rabbit IgG |
| Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb | 4511 | 20 µl | 43 kDa | Rabbit IgG |
| Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb | 8713 | 20 µl | 150 kDa | Rabbit IgG |
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb | 4370 | 20 µl | 44, 42 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Angiogenesis Antibody Sampler Kit provides an economical means to investigate the angiogenic pathway downstream of VEGFR2. The kit contains enough primary antibody to perform two western blots per primary antibody.
Storage
Background
Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Signaling from VEGFR2 is necessary for angiogenesis in vivo (2-4). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (5). Phosphorylation of VEGFR2 at Tyr1212 provides a docking site for GRB2 binding and phosphorylation at Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ (1,5,6). Activation of VEGFR2 during angiogenesis leads to signaling through multiple downstream kinase pathways including Akt, Src, FAK, p38, and Erk1/2 (2,7).
- Meyer, M. et al. (1999) EMBO J 18, 363-74.
- Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.
- Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.
- Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.
- Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.
- Takahashi, T. et al. (2001) EMBO J 20, 2768-78.
- Le Boeuf, F. et al. (2004) J Biol Chem 279, 39175-85.
Background References
Trademarks and Patents
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