Phospho-(Ser) CDKs Substrate Antibody (#2324) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, IP, E-P

REACTIVITY:

SENSITIVITY:

Endogenous

MW (kDa):

SOURCE:

Rabbit

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100
Peptide ELISA (DELFIA)	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-(Ser) CDKs Substrate Antibody detects phospho-serine in a (K/R)(S*)PX(K/R) motif. The antibody is phospho-specific but does not recognize phospho-serine in the absence of the CDK motif. The antibody does not cross-react with phospho-threonine- or phospho-tyrosine-containing peptides/proteins. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phospho-CDK substrate peptides . Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cyclin-dependent kinases (CDKs) are a family of Ser/Thr kinases that regulate cell-cycle transitions through their association and subsequent phosphorylation of targets in a strictly ordered fashion (1). The substrates for CDKs are proline-directed. The consensus amino acid sequence for CDK substrate is (K/R)(S*)PX(K/R), where X denotes any one of the 20 amino acids (2-4) and S* is the phosphorylation site. Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab^™ Rabbit mAb mix recognizes phosphorylated CDK substrates at their consensus motif, providing a powerful tool for CDK target discovery and characterization, as well as HTS drug screening for potential kinase regulators.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation E-P: Peptide ELISA (DELFIA)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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