Human Activated T Cell Markers Flow Cytometry Panel

All antibodies in this kit are compatible with the Flow Cytometry, Live Cell Protocol for Directly Conjugated Antibodies and can be used in a single staining mix. After antibody staining and prior to acquisition on a flow cytometer, we recommend adding Propidium Iodide or 7-AAD to enable identification and exclusion of dead cells from the analysis.

Gating strategy for identifying activated T cells:
If Propidium Iodide or 7-AAD was used, first gate on viable cells. Next, gate on lymphocytes based on forward scatter and side scatter. Helper T cells are the CD3+CD4+ cells within the lymphocyte gate. Activated helper T cells are positive for expression of CD69 and/or IL-2Rα/CD25. Regulatory T cells can also express IL-2Rα/CD25. Cytotoxic T cells are the CD3+CD8+ cells within the lymphocyte gate. Activated cytotoxic T cells are positive for expression of CD69 and/or IL-2Rα/CD25.

Antibodies are supplied in 10 mM NaH₂PO₄, 150 mM NaCl, 0.09% NaN₃, 0.1% gelatin, pH7.2. Store at 4° C. Do not aliquot the antibodies. Protect from light. Do not freeze.

All components in this kit are stable in accordance with the date printed on the outer packaging label when stored at the recommended temperature. Please refer to product labels, datasheets, or web pages for specific "Best By" dates for each individual component.

Each antibody in the Human Activated T Cell Markers Flow Cytometry Panel detects endogenous levels of its target protein. CD3 (UCHT1) Mouse mAb (PE Conjugate), CD4 (RPA-T4) Mouse mAb (violetFluor^™ 450 Conjugate), CD8α (RPA-T8) Mouse mAb (FITC Conjugate), IL2-Rα/CD25 (BC96) Mouse mAb (APC Conjugate), and CD69 (FN50) Mouse mAb (PE-Cy7^® Conjugate) detect epitopes within the extracellular domains.

The Human Activated T Cell Markers Flow Cytometry Panel can be used to identify activated human T cells.

T cells are identified by expression of CD3. There are two major subsets of conventional T cells: helper T cells which express CD4, and cytotoxic T cells which express CD8. Upon stimulation through the T cell receptor, T cells upregulate expression of CD69 and IL-2Rα/CD25. Regulatory T cells constitutively express IL-2Rα/CD25.