Background
Ionomycin is a potent and selective calcium ionophore agent (1,2). The molecule acts as a motile Ca2+ carrier and enhances Ca2+ influx by direct stimulation of store-regulated cation entry across biological membranes (3). At the micromolar level, ionomycin can activate Ca2+/Calmodulin dependent kinase and phosphatase to stimulate gene expression (4). In human T cells, ionomycin induces hydrolysis of phosphoinositides and activates PKC to mediate T cell activation (5). Ionomycin treatment of human B cells induces the activation of calcium-dependent endonuclease and results in apoptosis (6). Ionomycin treatment of bovine aortic endothelial cells (BAECs) induces rapid dephosphorylation of eNOS at Thr495 and mediates eNOS activation (7).
- Liu, C. and Hermann, T.E. (1978) J Biol Chem 253, 5892-4.
- Kauffman, R.F. et al. (1980) J Biol Chem 255, 2735-9.
- Morgan, A.J. and Jacob, R. (1994) Biochem J 300 ( Pt 3), 665-72.
- Lobo, F.M. et al. (1999) J Immunol 162, 2057-63.
- Chatila, T. et al. (1989) J Immunol 143, 1283-9.
- Aagaard-Tillery, K.M. and Jelinek, D.F. (1995) J Immunol 155, 3297-307.
- Lin, M.I. et al. (2003) J Biol Chem 278, 44719-26.
Molecular Formula
C41H70O9 • Ca2+
Molecular Weight
747.08 g/mol
Purity
>99%
CAS
56092-82-1
Solubility
Soluble in DMSO at 1.6mg/ml and EtOH at 20mg/ml.
Storage
Directions for Use
Ionomycin, calcium salt is supplied as 2.5 mg powder. Store at or below -20ºC. Before use, dissolve powder in 1.1 ml DMSO to make a 3 mM ionomycin stock solution. For working concentrations of 1 μM-3 μM, dilute DMSO stock 1:3000 to 1:1000. Treat cells with the desired concentration for intended time.
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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