Background
Calyculin A inhibits the activity of protein phosphatases PP1 and PP2A (1,2). Unlike Okadaic acid, which reduces PP2A activity but has little effect on PP1 activity, Calyculin A inhibits both phosphatases (1). Neither Calyculin A nor Okadaic acid inhibit acid or alkaline phosphatases or phospho- tyrosine protein phosphatases (2).
Description
Calyculin A is a more potent phosphatase inhibitor than Okadaic acid (2). As shown by Western blot, treatment of cells with 100 nM Calyculin A for 30 minutes induces threonine phosphorylation, detected by Phospho-Threonine-Polyclonal Antibody #9381. IC50 values for inhibitory activity against PP1 are approximately 2 nM. IC50 values for inhibitory activity against PP2A are approximately 0.5 -1.0 nM.
Molecular Formula
C50H81N4O15P
Molecular Weight
1009.17 g/mol
Purity
>98%
CAS
101932-71-2
Solubility
Soluble in DMSO at 50mM and EtOH at 1mg/ml.
Storage
Directions for Use
Calyculin A is supplied as a lyophilized clear film. For 10 µM stock, reconstitute in 1 ml DMSO. Store in aliquots at -20ºC in the dark. Treat cells with 50-100 nM calyculin A for 5-30 minutes. Store in aliquots tightly sealed (unopened) at -20ºC in the dark. See MSDS.
This compound is sold only for use in extremely dilute solutions for biological research. No other use is intended and any other use involves substantial hazards. This compound should never be handled in powder or aerosol form or in any other form susceptible to uncontrolled release in the laboratory, even in very small quantities.
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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