BMS-303141 (#89022) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Background

The small-molecule BMS-303141 is a potent (IC₅₀= 0.13 μM) inhibitor of ATP-citrate lyase (ACL). ACL catalyzes the formation of cytosolic acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for fatty acid and cholesterol biosynthesis. BMS-303141 also weakly inhibits acetyl-CoA carboxylase (ACC) isoforms ACC1 and ACC2. Treatment of high-fat fed mice with BMS-303141 reduced weight gain and decreased levels of plasma cholesterol, triglycerides, and glucose (1). BMS-303141 treatment of prostate cancer cells impaired cell proliferation or induced death in androgen-depleted, castration-resistant prostate cancer cells (2). Hepatocellular carcinoma cells responded to treatment with BMS-303141, reducing cell proliferation and promoting apoptosis. BMS-303141 is thought to induce endoplasmic reticulum (ER) stress and activate the p-eIF2α/ATF4/CHOP pathway to promote apoptosis. The combined treatment of a mouse xenograft model with BMS-303141 and a protein kinase inhibitor reduced tumor volume and weight (3).

Molecular Formula

C₁₉H₁₅Cl₂NO₄S

Molecular Weight

424.3 g/mol

Purity

>98%

CAS

943962-47-8

Solubility

Soluble in DMSO at 25 mg/mL or ethanol at 20 mg/mL.

Storage

Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Directions for Use

BMS-303141 is supplied as a lyophilized powder. For a 15 mM stock, reconstitute 5 mg of powder in 0.79 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Background References

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明，且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的，或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的，或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专