Background
17-AAG is a semi-synthetic derivative of geldanamycin, demonstrating greater stability than its parent compound (1). It binds specifically to heat shock protein HSP90 in a manner similar to geldanamycin, but with weaker binding (1). Through specific binding with HSP90, 17-AAG has been shown to decrease levels of many proteins including androgen receptor (AR), HER2, and Akt, while increasing levels of HSP90 in prostate cancer cell lines (2). 17-AAG binds to a conserved pocket in the HSP90 family of chaperone proteins and this occupation causes the degradation of several signaling proteins (2).
Molecular Formula
C31H43N3O8
Molecular Weight
585.7 g/mol
Purity
>98%
CAS
75747-14-7
Solubility
Soluble in DMSO at 50mg/ml or EtOH at 5mg/ml.
Storage
Directions for Use
17-AAG is supplied as a lyophilized powder. For a 1 mM stock, reconstitute the 500 μg in 853.7 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 0.1-1 μM for 4-24 hours. Soluble in DMSO at 150 mg/mL; soluble in ethanol at 5 mg/mL. Maximum solubility in plain water estimated to be about 20-50 μM.
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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