Revision 1

#89984

Store at -20C

Ym1/Chi3l3 (F7T7N) Rabbit mAb
CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W

Reactivity:
M

Sensitivity:
Endogenous

MW (kDa):
44

Source/Isotype:
Rabbit IgG

UniProt ID:
#O35744

Entrez-Gene Id:
12655

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

Ym1/Chi3l3 (F7T7N) Rabbit mAb recognizes endogenous levels of total Ym1/Chi3l3 protein. This antibody does not cross-react with Ym2/Chi3l4 or AMCase/Chia proteins. This antibody detects a 28 kDa protein of unknown identity in some cell lines and tissues.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp275 of mouse Ym1/Chi3l3 protein.

Background

Chitinase-like protein 3 (CHI3L3), also called Ym1, is a rodent specific chitinase-like protein (CLP) (1,7). Chitinases are a class of chitin-degrading enzymes produced in the host, which play a protective role in innate immunity against the chitin-containing pathogens, including parasites, fungi, and arthropods (1,2,7). In healthy tissues, Ym1 is expressed in the spleen, bone marrow, and lung (7). Ym1 expression can be induced by various inflammatory stimuli, such as allergic airway inflammation, parasitic infection, or autoimmune disease (7). Ym1 is primarily produced by alternatively activated (M2) macrophages and is often used as a marker for these cells (3-7).

Background References

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting

Cross-Reactivity Key

M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

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Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

#89984

Ym1/Chi3l3 (F7T7N) Rabbit mAb

Cell Signaling Technology Logo
Western blot analysis of extracts from mouse bone marrow, lung, and brain tissues using Ym1/Chi3l3 (F7T7N) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of Ym1/Chi3l3 protein in mouse brain tissue is consistent with the predicted expression pattern.
Western Blotting Image 1: Ym1/Chi3l3 (F7T7N) Rabbit mAb
Western blot analysis of mouse peritoneal macrophages, untreated (-) or treated with Lipopolysaccharides (LPS) #14011 and Mouse IL-4 Recombinant Protein #40004 (mIL-4) (1 μg/mL and 12.5 ng/mL, 24 hr; +), using Ym1/Chi3l3 (F7T7N) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Ym1/Chi3l3 is induced by LPS and mIL-4 treatment as expected.
Western Blotting Image 2: Ym1/Chi3l3 (F7T7N) Rabbit mAb
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length mouse Ym1/Chi3l3 protein (mYm1/Chi3l3-Myc/DDK; +), Myc/DDK-tagged full-length mouse Ym2/Chi3l4 protein (mYm2/Chi3l4-Myc/DDK; +), or Myc/DDK-tagged full-length mouse AMCase/Chia protein (mAMCase/Chia-Myc/DDK; +), using Ym1/Chi3l3 (F7T7N) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 3: Ym1/Chi3l3 (F7T7N) Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.