Wnt/β-Catenin Activated Targets Antibody Sampler Kit

1 Kit

(8 x 20 microliters)

Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

[email protected]

cellsignal.com

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes	Product #	Quantity	Mol. Wt	Isotype/Source
CD44 (156-3C11) Mouse mAb	3570	20 µl	80 kDa	Mouse IgG2a
Cyclin D1 (92G2) Rabbit mAb	2978	20 µl	36 kDa	Rabbit IgG
c-Jun (60A8) Rabbit mAb	9165	20 µl	43, 48 kDa	Rabbit IgG
LEF1 (C12A5) Rabbit mAb	2230	20 µl	25-58 kDa	Rabbit IgG
Met (D1C2) XP^® Rabbit mAb	8198	20 µl	140, 170 kDa	Rabbit IgG
MMP-7 (D4H5) XP^® Rabbit mAb	3801	20 µl	28, 20-22 kDa	Rabbit
c-Myc (D84C12) Rabbit mAb	5605	20 µl	57-65 kDa	Rabbit IgG
TCF1/TCF7 (C63D9) Rabbit mAb	2203	20 µl	48, 50 kDa	Rabbit IgG
Anti-mouse IgG, HRP-linked Antibody	7076	100 µl		Horse
Anti-rabbit IgG, HRP-linked Antibody	7074	100 µl		Goat

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Wnt/β-Catenin Activated Targets Antibody Sampler Kit provides an economical means to investigate target proteins of the Wnt/β-Catenin signaling pathway. This kit contains enough primary antibody to perform two western blots per primary.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

The Wnt family includes several secreted glycoproteins that play important roles in animal development. β-catenin is a key downstream effector of the Wnt signaling pathway that research studies have shown is implicated in early embryonic development and tumorigenesis in vertebrates (1-3). Following binding of Wnt family proteins to the Frizzled receptor, β-catenin translocates to the nucleus where it interacts with LEF1 and TCF1 to activate canonical targets (4). Accepted canonical targets include CD44, cyclin D1, c-Jun, c-Myc, Met, and MMP-7 (5-11).

Background References

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com

For Research Use Only. Not for Use in Diagnostic Procedures.