Cat. # | Size | Price | Inventory |
---|---|---|---|
8334T | 1 Kit (5 x 20 microliters) |
Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
---|---|---|---|---|---|
Phospho-HSL (Ser563) Antibody 4139 | 20 µl |
|
M | 81, 83 | Rabbit |
Phospho-HSL (Ser565) Antibody 4137 | 20 µl |
|
M | 81, 83 | Rabbit |
Perilipin-1 (D1D8) XP® Rabbit mAb 9349 | 20 µl |
|
H M | 62 | Rabbit IgG |
Anti-rabbit IgG, HRP-linked Antibody 7074 | 100 µl |
|
Goat | ||
HSL (D6W5S) XP® Rabbit mAb 18381 | 20 µl |
|
H M | 81, 83 | Rabbit IgG |
Phospho-HSL (Ser660) Antibody 45804 | 20 µl |
|
H M | 81, 83 | Rabbit |
Product Information
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser552 of human HSL (equivalent to Ser563 of rat HSL), Ser554 of human HSL (equivalent to Ser565 of rat HSL), Ser651 of mouse HSL (equivalent to Ser660 of rat HSL), or the sequence of human HSL. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues around Ile419 of human perilipin protein.
Triacylglycerol is stored in lipid droplets as a primary energy reserve. During lipolysis, triacylglycerols in adipocytes are hydrolyzed into free fatty acids and glycerol. Perilipin, localized at the periphery of lipid droplets, serves as a protective coating against lipases (1-3). Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin and hormone-sensitive lipase (HSL) (1,2,4,5). Phosphorylation of perilipin results in the conformational change that exposes lipid droplets to endogenous lipases, such as HSL (2). Phosphorylation of HSL at Ser563, Ser659, and Ser660 by PKA stimulates HSL activity, which in turn catalyzes the hydrolysis of triacylglycerol (6,7).
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