Cat. # | Size | Price | Inventory |
---|---|---|---|
3739S | 100 µl (50 tests) |
REACTIVITY | All |
SENSITIVITY | Transfected Only |
MW (kDa) | |
Source/Isotype | Mouse IgG2a kappa |
Product Information
Application | Dilution |
---|---|
Flow Cytometry (Fixed/Permeabilized) | 1:50 |
All reagents required for this protocol may be efficiently purchased together in our Intracellular Flow Cytometry Kit (Methanol) #13593, or individually using the catalog numbers listed below.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: When including fluorescent cellular dyes in your experiment (including viability dyes, DNA dyes, etc.), please refer to the dye product page for the recommended protocol. Visit www.cellsignal.com for a full listing of cellular dyes validated for use in flow cytometry.
NOTE: Adherent cells or tissue should be dissociated and in single-cell suspension prior to fixation.
NOTE: Optimal centrifugation conditions will vary depending upon cell type and reagent volume. Generally, 150-300g for 1-5 minutes will be sufficient to pellet the cells.
NOTE: If using whole blood, lyse red blood cells and wash by centrifugation prior to fixation.
NOTE: Antibodies targeting CD markers or other extracellular proteins may be added prior to fixation if the epitope is disrupted by formaldehyde and/or methanol. The antibodies will remain bound to the target of interest during the fixation and permeabilization process. However, note that some fluorophores (including PE and APC) are damaged by methanol and thus should not be added prior to permeabilization. Conduct a small-scale experiment if you are unsure.
NOTE: Count cells using a hemocytometer or alternative method.
posted July 2009
revised June 2020
实验步骤编号:407
预期的所有物种
使用与人 c-Myc (EQKLISEEDL) 残基410-419相对应的合成肽对动物进行免疫接种来产生单克隆抗体。
在使用免疫印迹法、免疫沉淀法和免疫染色技术对蛋白进行标记和检测时,表位标签十分有用。由于分子量小,它们不太可能对标记蛋白的生化特性造成影响。
Myc 表位标签广泛用于检测细菌、酵母、昆虫和哺乳动物细胞系中重组蛋白的表达。
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