Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose® Bead Conjugate) (#3423) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

Concentration:

5000 bead

SENSITIVITY:

Endogenous

Source/Isotype:

Rabbit IgG

Product Information

Product Usage Information

Directions for Use: Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.

Application	Dilution
Immunoprecipitation	1:20

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Specificity / Sensitivity

Rabbit (DA1E) mAb IgG XP^® Isotype Control is not directed against any known antigen. It functions as a control for rabbit IgG monoclonal antibodies.

Product Description

Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (whole molecule) from Cell Signaling Technology is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose^® beads. Rabbit (DA1E) mAb IgG XP^® Isotype Control (Sepharose^® Bead Conjugate) is useful to determine non-specific immunoprecipitation complexes.

Background

Control antibodies are used to estimate the non-specific binding of target primary antibodies due to F_c receptor binding or other protein-protein interactions.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IP: Immunoprecipitation

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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