Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (Sepharose^® Bead Conjugate)

Product Usage Information

Solutions and Reagents

Storage Buffer:20 mM Tris-HCl (pH 7.4)150 mM NaCl1 mM Na₂EDTA1 mM EGTA1% Triton X-1002.5 mM sodium pyrophosphate1 mM β-glycerophosphate1 mM Na₃VO₄1 µg/ml leupeptin50% glycerolNote: Please aliquot prior to first use.

Storage

Specificity / Sensitivity

Species Reactivity:

Human, Mouse, Rat, Monkey, S. cerevisiae

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr180/Tyr182 of human p38 MAP kinase.

Background

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).