Revision 1
#91256
Store at -20C
CD206/MRC1 (E2L9N) & CO-0035-750 SignalStar™ Oligo-Antibody Pair
1 Kit
(10 slides)
UniProt ID:
#P22897
Entrez-Gene Id:
#4360
Orders:
877-616-CELL (2355)
Support:
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
| Product Includes | Product # | Volume | Reactivity | Isotype |
|---|---|---|---|---|
| CD206/MRC1 (E2L9N) Rabbit mAb (SignalStar™ Conjugate 0035) | 90922 | 50 µl | H | Rabbit IgG |
| Complementary Oligo (CO-0035-750) | 26850 | 22 µl |
Storage
SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.
Description
SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
Specificity/Sensitivity
CD206/MRC1 (E2L9N) Rabbit mAb (SignalStar™ Conjugate 0035) recognizes endogenous levels of total CD206/MRC1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human CD206/MRC1 protein.
Background
The mannose receptor (MR/CLEC13D/CD206/MMR/MRC1/Macrophage mannose receptor 1) is an endocytic receptor expressed by populations of dendritic cells, macrophages, and nonvascular endothelium (1). The mannose receptor is a heavily glycosylated type I transmembrane protein with three types of extracellular domains and a short carboxy-terminal cytoplasmic domain with no apparent signaling motif (2-4). The extracellular portion of the protein is made up of a CR domain, which binds sulfated glycans, an FNII domain, which binds collagens, and eight C-type lectin domains, which bind carbohydrates containing mannose, fucose, or GlcNAc (4-7). The receptor recycles between the plasma membrane and early endosomes (8). Functions include a role in antigen cross-presentation, clearance of endogenous proteins, pathogen detection, and trafficking through lymphatic vessels (9-12).
Background References
- Martinez-Pomares, L. (2012) J Leukoc Biol 92, 1177-86.
- Lennartz, M.R. et al. (1989) J Biol Chem 264, 2385-90.
- Wileman, T.E. et al. (1986) Proc Natl Acad Sci U S A 83, 2501-5.
- Taylor, M.E. et al. (1990) J Biol Chem 265, 12156-62.
- Fiete, D.J. et al. (1998) Proc Natl Acad Sci U S A 95, 2089-93.
- Napper, C.E. et al. (2006) Biochem J 395, 579-86.
- Fiete, D. et al. (1997) Proc Natl Acad Sci U S A 94, 11256-61.
- Tietze, C. et al. (1982) J Cell Biol 92, 417-24.
- Burgdorf, S. et al. (2006) J Immunol 176, 6770-6.
- Lee, S.J. et al. (2002) Science 295, 1898-901.
- Milone, M.C. and Fitzgerald-Bocarsly, P. (1998) J Immunol 161, 2391-9.
- Marttila-Ichihara, F. et al. (2008) Blood 112, 64-72.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: Human
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStar is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品 ， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] •
Web:
cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.
Revision 1
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using CD206/MRC1 (E2L9N) & CO-0035-488 SignalStar™ Oligo-Antibody Pair #99626 (green), Pan-Keratin (C11) & CO-0003-488 SignalStar™ Oligo-Antibody Pair #63566 (cyan), CD8α (D8A8Y) & CO-0004-594 SignalStar™ Oligo-Antibody Pair #19166 (red), CD31 (PECAM-1) (89C2) & CO-0028-594 SignalStar™ Oligo-Antibody Pair #33420 (yellow), Ki-67 (8D5) & CO-0014-647 SignalStar™ Oligo-Antibody Pair #32103 (magenta), and ProLong Gold Antifade Reagent with DAPI #8961 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using CD206/MRC1 (E2L9N) & CO-0035-488 SignalStar™ Oligo-Antibody Pair #99626 (green). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ immunohistochemical analysis of paraffin-embedded human colorectal adenocarcinoma using CD206/MRC1 (E2L9N) & CO-0035-594 SignalStar™ Oligo-Antibody Pair #52522 (yellow). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] •
Web:
cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.
Revision 1
SignalStar™ immunohistochemical analysis of paraffin-embedded human colorectal adenocarcinoma using CD206/MRC1 (E2L9N) & CO-0035-647 SignalStar™ Oligo-Antibody Pair #75931 (red). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using CD206/MRC1 (E2L9N) & CO-0035-750 SignalStar™ Oligo-Antibody Pair #91256 (cyan). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using CD206/MRC1 (E2L9N) & CO-0035-488 SignalStar™ Oligo-Antibody Pair #99626 (left, green) and ProLong Gold Antifade Reagent with DAPI #8961 (left, blue) compared to chromogenic immunohistochemical analysis of a serial section of paraffin-embedded human squamous cell lung carcinoma using CD206/MRC1 (E2L9N) Rabbit mAb #91992 (right). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] •
Web:
cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.
Revision 1
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human colorectal adenocarcinoma using CD206/MRC1 (E2L9N) & CO-0035-647 SignalStar™ Oligo-Antibody Pair #75931 (red) and CD11c (D3V1E) & CO-0017-750 SignalStar™ Oligo-Antibody Pair #17879 (cyan). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using Pan-Keratin (C11) & CO-0003-488 SignalStar™ Oligo-Antibody Pair #63566 (cyan), SIRPα/SHPS1 (D6I3M) & CO-0034-647 SignalStar™ Oligo-Antibody Pair #80150 (red), CD206/MRC1 (E2L9N) & CO-0035-488 SignalStar™ Oligo-Antibody Pair #99626 (green), and CD45 (Intracellular Domain) (D9M8I) & CO-0013-647 SignalStar™ Oligo-Antibody Pair #32740 (magenta). All fluorophores have been assigned a pseudocolor, as indicated.
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] •
Web:
cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.