Revision 9

#4418

Store at +4C

Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate)
CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
IF-F, IF-IC, FC-FP, FC-L

Reactivity:
R

Source/Isotype:
Goat

Product Usage Information

The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:500 - 1:2000 should yield acceptable results for immunofluorescent and flow cytometry assays.

Storage

Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity/Sensitivity

IgG is prepared from goat antibodies that have been adsorbed against mouse IgG, mouse serum and human serum. Cross-reactivity with mouse primary antibodies has been observed.

Description

Anti-rat IgG (H+L) was conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and formulated at 2 mg/ml.

Background

This product has been optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized) FC-L: Flow Cytometry (Live)

Cross-Reactivity Key

R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for Immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 9

#4418

Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate)

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of mouse cerebellum using CD44 (IM7) Rat mAb #39037 detected with Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate) #4418 (red) and Calbindin (D1I4Q) XP® Rabbit mAb #13176 detected with Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 (green). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 1: Anti-rat IgG (H+L), (Alexa Fluor<sup>®</sup> 647 Conjugate)
Confocal immunofluorescent analysis of HeLa cells using ORC2 (3G6) Rat mAb #4736 detected with Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate) (blue) and COX IV (3E11) Rabbit mAb #4850 detected with Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Immunofluorescence Image 1: Anti-rat IgG (H+L), (Alexa Fluor<sup>®</sup> 647 Conjugate)
Flow cytometric analysis of Jurkat cells using SH2D1A (XLP 1D12) Rat mAb #2805 (green solid line) compared to secondary antibody alone (green dashed line) or unstained cells (orange dashed line). Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate) was used as a secondary antibody.
Flow Cytometry Image 1: Anti-rat IgG (H+L), (Alexa Fluor<sup>®</sup> 647 Conjugate)
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.