Anti-Human IgA, alpha-Chain Specific, HRP-Linked Antibody (#80403) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

REACTIVITY:

SENSITIVITY:

MW (kDa):

Source/Isotype:

Goat

Product Information

Product Usage Information

Reconstitute with 2.0 ml of dH₂0 to create a 0.8 mg/ml working solution. Centrifuge if the solution is not clear. It is recommended to prepare the working solution on day of use.

Recommended Antibody Dilutions:

ELISA (chromogenic substrates): 1:5K-1:100K

Western Blotting (chromogenic substrates): 1:5K - 1:100K

Western Blotting (ECL substrates): 1:10K - 1:200K

Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.

Storage

Supplied lyophilized with a formulation of 0.01M Sodium Phosphate (pH 7.6), 0.25M NaCl, and 15 mg/ml bovine serum albumin (BSA). Store lyophilized at 4ºC. In lyophilized form, the product is stable for 24 months. Once in solution, store at -80ºC or add glycerol (ACS grade or better) to a final concentration of 50% and store at -20ºC. Solutions should be used within 12 months. Aliquot to avoid multiple freeze/thaw cycles.

Specificity / Sensitivity

Anti-Human IgA, alpha-Chain Specific, HRP-Linked Antibody detects the heavy chain of human IgA but not human IgG or IgM. This antibody does not detect other non-immunoglobulin serum proteins. It may cross-react with IgA from other species.

Species Reactivity:

Human

Source / Purification

Anti-Human IgA, alpha-Chain Specific, HRP-Linked Antibody is produced by immunizing goats with human IgA. The Anti-Human IgA, alpha-Chain is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Product Description

Affinity purified goat Anti-Human IgA, alpha-Chain Specific antibody is conjugated to horseradish peroxidase (HRP). This product has been optimized for use as a secondary antibody in Western blotting and ELISA applications.

Background

Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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