PTMScan® Lys-C Protease (#39003) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Information

Product Usage Information

For PTMScan^® protocols we recommend making a 5 mg/ml stock solution by resuspending 1.2 mg in 0.24 ml of 20 mM HEPES pH 8.0, aliquot for single use and store at -80°C. See the PTMScan^® protocol for further details.

Storage

Store lyophilized Lys-C at -20°C. Once reconstituted, the enzyme can be aliquoted and stored at -80°C to help avoid damaging freeze thaw cycles. Lyophilized Lys-C has a shelf life of 1 year at -20°C and solutions are stable for 6 months at -80°C.

Specificity / Sensitivity

The activity of Lys-C is greater than or equal to 3 AU/mg.

Source / Purification

Lys-C was produced recombinantly in E. coli, purified and is provided lyophilized in 25 mM Tris-HCl and mannitol. Purity is >90% by SDS-PAGE. One amidase unit (AU) is the amount of enzyme that produces 1 micromole of Ac-Lys-pNA hydrochloride salt per minute at pH 9.5 at 30°C.

Product Description

Lysl-endopeptidase (Lys-C) hydrolyzes amide and peptide ester bonds on the carboxyl side of lysine residues and hydrolyzes S-aminoethylcysteine residues.

Background

Lysl-endopeptidase (Lys-C) cleaves proteins on the carboxyl side of lysine residues. This makes it a useful tool in protein sequencing and proteomic applications. The enzyme was originally purified from the Gram-negative soil bacteria Achromobacter lyticus (1). PTMScan^® Lys-C is produced recombinantly in E. coli. It has been found to work optimally at the temperatures of 30-37°C and pH ranges from 9.0-9.5 but will undergo degradation at temperatures of 50°C and higher. Activity is maintained in 4M urea or 0.1% SDS solutions for up to 6 hours at 30°C. Inhibitors of the enzyme include PMSF, DFP, and TLCK.

Soejima, M. and Masaki, T. (1984) Tanpakushitsu Kakusan Koso 29, 1532-7.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PTMScan is a registered trademark of Cell Signaling Technology, Inc.

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