Revision 4

#9812Store at -20C

1 个试剂盒

(16 x 40 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Products Included Motif Quantity Isotype
Phospho-Akt Substrate (110B7E) Rabbit mAb #9614 RXX(S*/T*) 40 µl Rabbit IgG
Phospho-Akt Substrate (23C8D2) Rabbit mAb #10001 RXRXX(S*/T*) 40 µl Rabbit IgG
Phospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb #5759 (L/M)XRXX(S*/T*), RXX(S*/T*) 40 µl Rabbit IgG
Phospho-(Ser) ATM/ATR Substrate (D23H2/D69H5) Rabbit mAb #9607 S*Q 40 µl Rabbit IgG
Phospho-(Ser/Thr) ATM/ATR Substrate (S*/T*QG) (P-S/T2-100) Rabbit mAb #6966 (S*/T*)QG, (S*/T*)Q 40 µl Rabbit IgG
Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb #9477 (K/H)S*P 40 µl Rabbit IgG
Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb #8738 (S*/T*)DXE 40 µl Rabbit IgG
Phospho-MAPK/CDK Substrates (34B2) Rabbit mAb #2325 PXS*P, S*PX(K/R) 40 µl Rabbit IgG
Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb #9634 (F/K)XX(F/Y)(S*/T*)(F/Y) 40 µl Mouse IgG2a
Phospho-PKA Substrate (100G7E) Rabbit mAb #9624 (K/R)(K/R)X(S*/T*) 40 µl Rabbit IgG
Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb #6967 (K/R)XS*X(K/R) 40 µl Rabbit IgG
Phospho-(Thr) PLK Binding Motif (D73F6) Rabbit mAb #5243 ST*P 40 µl Rabbit IgG
Phospho-Thr-Pro Motif Antibody #3003 † T*P, T*PP 40 µl Rabbit IgG
Phospho-Thr-Pro-Glu (C32G12) Rabbit mAb #3004 T*PE, T*P 40 µl Rabbit IgG
Phospho-Thr-X-Arg Motif Antibody #2351 T*X(K/R) 40 µl Rabbit IgG
Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb #8954 Y* 40 µl Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat
Anti-mouse IgG, HRP-linked Antibody #7076 100 µl Horse

Description

The KinomeView® Profiling Kit provides a set of Phospho-Motif Antibodies that cover a large portion of the kinome and react broadly with serine, threonine, and tyrosine phosphorylation mediated by diverse kinase families throughout the kinome. This kit will provide researchers with an immunoblotting strategy to dissect the complexity of the phosphoproteome and determine the kinase families involved in the regulation of diverse physiological processes. By using the provided Phospho-motif Antibodies, the investigator can assess global changes in protein phosphorylation by western analysis across a range of experimental samples and conditions.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Cell Signaling Technology (CST) has developed Phospho-motif Antibodies that have broad immunoreactivity for serine, threonine and tyrosine phosphorylation mediated by both known and unknown kinases. Using these Motif Antibodies for western blotting provides a Kinome-wide view of cellular phosphorylation.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

    限制使用

    除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。

    专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品, (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

    Revision 4
    #9812

    KinomeView® Profiling Kit

    KinomeView® Profiling Kit: Image 1 Expand Image
    使用 Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb #8738,对未经处理或经 Calyculin A #9902(10 nM,30 分钟)或 Staurosporine #9953(1 μM,3 小时)处理的 HeLa 细胞提取物进行蛋白质印迹分析。
    KinomeView® Profiling Kit: Image 2 Expand Image
    使用 Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb #9477,对细胞周期 G1/S、G2/M 和 G0 期同步处理的 COS-7 细胞提取物进行蛋白质印迹分析。
    KinomeView® Profiling Kit: Image 3 Expand Image
    磷酸酪氨酸 (Tyr) 抗体与 Tyr-磷酸化蛋白质发生广泛反应。HeLa 细胞经过不同条件处理,一旦发生通过 EGF 处理的 EGF 受体刺激,即可观察到 Tyr 磷酸化的诱导;EGFR 介导的磷酸化受到酪氨酸激酶抑制剂 Gefitinib 的抑制。
    KinomeView® Profiling Kit: Image 4 Expand Image
    磷酸化 MAPK/CDK 底物抗体与在 Ser 残基上发生磷酸化且 Ser 邻近的 -2 和 +1 位为 Pro 残基的蛋白质有广谱的反应活性。CMGC 家族激酶包括丝裂原活化蛋白激酶 (MAPK) 和细胞周期蛋白依赖性激酶 (CDK),可对含该基序的底物进行磷酸化。通过 Nocodazole 介导的有丝分裂停滞检测到该基序磷酸化的变化,以及通过 HeLa 细胞中 U0126 的 MEK/ERK 信号转导抑制。
    KinomeView® Profiling Kit: Image 5 Expand Image
    使用 KinomeView 表达谱分析试剂盒组分抗体,对未经处理或按指定处理的各种细胞类型的提取物进行蛋白质印迹分析。选择缩写:TBB,CKII 抑制剂 4,5,6,7-四溴苯并三唑;PDBu,佛波醇 12,13-二丁酸酯。