Product Information
Product Usage Information
Cells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase, solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan® antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS/MS analysis. CST recommends the use of PTMScan® IAP Buffer #9993 included in the kit. An alternate PTMScan® IAP Buffer Plus Detergent #9992 which may reduce nonspecific interactions is available separately. A detailed protocol and Limited Use License allowing the use of the patented PTMScan® method is included with the kit.
Storage
Product Description
Background
For more information on our PTMScan® Motif Antibody Kits, please visit www.cellsignal.com. Specific product background information can be found on the web page for each PTMScan® Motif Antibody kit component. Cleaved Caspase Substrate Motif background is described on the webpage for PTMScan® Cleaved Caspase Substrate Motif [DE(T/S/A)D] Kit #12810. Phospho-MAPK/CDK Substrate Motif background is described on the webpage for PTMScan® Phospho-MAPK/CDK Substrate Motif (PXS*P and S*PXK/R) Kit #4652. Phospho-MAPK Substrate Motif background is described on the webpage for PTMScan® Phospho-MAPK Substrate Motif [PXpTP] Kit #14990. Phospho-Akt Substrate Motif background is described on the webpage for PTMScan® Phospho-Akt Substrate Motif mAb 2 (RXRXXS*/T*) Kit #5563. Phospho-ATM/ATR Substrate Motif background is described on the webpage for PTMScan® Phospho-ATM/ATR Substrate Motif [pSQ] Kit #12267.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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