Revision 5
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
:

:

:

:

Product Information

Product Usage Information

PTMScan® Trypsin, Mass Spectrometry Grade is provided for use with Cell Signaling Technology's patented PTMScan® protocol in the Trypsin Digestion of Enriched Peptides step. Reconstitute the lyophilized trypsin with 40ul of mass spectrometry grade water to make a 0.5 ug/ul stock. Use immediately or aliquot and store at -80°C. Consult the specific PTMScan® kit and protocol for more details.

Storage

Store lyophilized trypsin powder at -20ºC protected from moisture. Once reconstituted, store trypsin solutions at -80ºC. Lyophilized trypsin has a shelf life of 1 year at -20ºC and solutions are stable for 6 months at -80ºC.

Source / Purification

Isolated from bovine (Bos taurus) pancreas.

Product Description

Trypsin is a serine endopeptidase derived from its inactive pancreatic zymogen, trypsinogen, when the N-terminal 6 amino acid leader sequence is enzymatically removed. Activated trypsin cleaves amide and ester bonds of lysine and arginine and this mass spectrophotometry grade Trypsin is intended for use in protein sequencing, and proteomics applications.

Background

Trypsin digests polypeptides by hydrolysis at the carboxyl side of unmodified arginine and lysine residues (1). Proteolysis is slower when the cleavage site is flanked by acidic residues and will not occur if the lysine or arginine is followed by a proline (2-5). Trypsin's activity is optimal at pH 8.0 and is inhibited to varying degrees by organophosphorus compounds such as diisopropyl fluorophosphate, as well as EDTA, aprotinin, Ag+, and benzamidine (6-8).

  1. Brown, W.E. and Wold, F. (1973) Biochemistry 12, 828-34.
  2. Rodriguez, J. et al. (2008) J Proteome Res 7, 300-5.
  3. Leiros, H.K. et al. (2004) Protein Sci 13, 1056-70.
  4. Rawlings, N.D. and Barrett, A.J. (1994) Methods Enzymol 244, 19-61.
  5. Perona, J.J. and Craik, C.S. (1995) Protein Sci 4, 337-60.
  6. KOSTKA, V. and CARPENTER, F.H. (1964) J Biol Chem 239, 1799-803.
  7. Levilliers, N. et al. (1970) Arch Biochem Biophys 140, 474-83.
  8. Polgár, L. (2005) Cell Mol Life Sci 62, 2161-72.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PTMScan is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
仅供研究使用。不得用于诊断流程。