WB
H
Endogenous
180, 210
Rabbit IgG
#O60315
9839
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro722 of human ZEB2 protein.
Background
ZEB family proteins are zinc-finger and homeobox domain containing transcription factors. There are two members in mammals, ZEB1 (δ-EF1, TCF8, AREB6) and ZEB2 (SIP1). ZEB1 and ZEB2 contain two separate zinc-finger domains and a homeodomain (1). While ZEB proteins mainly function as transcriptional suppressors, they are able to activate transcription, dependent on DNA-context and cell type (1). One of the targets suppressed by ZEB proteins is E-cadherin. Downregulation of E-cadherin is one of the hallmarks of epithelial mesenchymal transition (EMT), a critical feature of normal embryonic development, which is also utilized by malignant epithelial tumors to spread beyond their origin (2-4). ZEB1 mutations are associated with posterior corneal dystrophy, and ZEB2 mutations were reported to be associated with Hirschsprung (HSCR) disease (5-8).
- Vandewalle, C. et al. (2009) Cell Mol Life Sci 66, 773-87.
- Aigner, K. et al. (2007) Oncogene 26, 6979-88.
- Peinado, H. et al. (2007) Nat Rev Cancer 7, 415-28.
- Moreno-Bueno, G. et al. (2008) Oncogene 27, 6958-69.
- Krafchak, C.M. et al. (2005) Am J Hum Genet 77, 694-708.
- Aldave, A.J. et al. (2007) Am J Med Genet A 143A, 2549-56.
- Dastot-Le Moal, F. et al. (2007) Hum Mutat 28, 313-21.
- Garavelli, L. and Mainardi, P.C. (2007) Orphanet J Rare Dis 2, 42.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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