WB, IP
H
Endogenous
78
Rabbit IgG
#Q9C0B5
25921
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu330 of human ZDHHC5 protein.
Background
Zinc finger DHHC domain-containing protein 5 (ZDHHC5) is a multipass membrane protein that is localized predominantly at the plasma membrane and endosomal compartment membranes (1). ZDHHC5 belongs to a large family of enzymes known as palmitoyl-acyltransferases. Using long-chain fatty acids such as palmitic acid, ZDHHC5 catalyzes the reversible, post-translational modification of cysteine residues within protein substrates to regulate their trafficking and subcellular localization (1,2). Research studies have shown that ZDHHC5 is itself regulated by multiple types of post-translational modifications, such as phosphorylation and palmitoylation, which regulate its subcellular localization and enzymatic activity (3,4). Palmitoylation mediated by ZDHHC5 plays a role in the host response to pathogens. Indeed, ZDHHC5-mediated palmitoylation of NOD1/2 pattern recognition receptors facilitates their correct localization to endosomal vesicle membranes containing internalized bacteria in order to initiate innate immune signaling cascades (5). Furthermore, ZDHHC5 was recently shown to interact with SARS-CoV-2 spike protein. This interaction is posited to facilitate S-acylation of the spike protein and fusion with host cell membranes (6,7).
- Ohno, Y. et al. (2006) Biochim Biophys Acta 1761, 474-83.
- Korycka, J. et al. (2012) Eur J Cell Biol 91, 107-17.
- Hao, J.W. et al. (2020) Nat Commun 11, 4765.
- Woodley, K.T. and Collins, M.O. (2019) EMBO Rep 20, e47472.
- Lu, Y. et al. (2019) Science 366, 460-467.
- Gordon, D.E. et al. (2020) Nature 583, 459-468.
- Wu, Z. et al. (2021) Signal Transduct Target Ther 6, 231.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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