WB, IP, ChIP
H M R Mk
Endogenous
95
Rabbit IgG
#O43167
9841
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro569 of human ZBTB24 protein.
Background
Zinc finger and BTB domain-containing protein 24 (ZBTB24), also called ZNF450, is a DNA-binding protein initially found to be expressed in response to BMP-2 signaling (1). ZBTB24 along with CDCA7 play a role in DNA methylation by recruiting the HELLS chromatin remodeling complex to centromeric satellite repeats (2). Mutations in ZBTB24 are linked to immunodeficiency and centromeric instability, and facial anomalies syndrome type 2 (ICF2) (3,4). ZBTB24 is recruited to DNA breaks by PARP1 and promotes assembly of the LIG4/XRCC4 complex, resulting in error free non-homologous end joining (NHEJ) (5). ZBTB24 has also been shown to play a role in trophoblast proliferation through the methylation of E-cadherin (6)
- Edgar, A.J. et al. (2005) J Cell Biochem 94, 202-15.
- Hardikar, S. et al. (2020) Protein Cell 11, 214-218.
- de Greef, J.C. et al. (2011) Am J Hum Genet 88, 796-804.
- Chouery, E. et al. (2012) Clin Genet 82, 489-93.
- Helfricht, A. et al. (2020) J Exp Med 217, e20191688. doi: 10.1084/jem.20191688.
- Ruan, H. et al. (2022) Bioengineered 13, 2777-2790.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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