WB, IP
H M R Mk
Endogenous
110
Rabbit IgG
#Q9H0D6
22803
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human XRN2 protein.
Background
5'-3' exoribonuclease 2 (XRN2) is a nuclear exonuclease that degrades RNA containing a 5’-monophosphate to component mononucleotides. XRN2 also plays an important role in the termination of transcription at the 3’-end of genes by displacing RNA polymerase II (RNAPII) from the DNA strand (1,2). According to the ‘torpedo’ model of transcription termination, XRN2 gains access to the 5’ phosphate of the nascent RNA during co-transcriptional polyadenylation site cleavage. XRN2 degrades RNA at a faster rate than RNAPII-mediated RNA synthesis, resulting in the eviction of RNAPII from the template (3-5). In addition, XRN2 is essential for maturation of 5.8S and 28S ribosomal RNA and small nucleolar RNA molecules (2). Several research studies suggest that XRN2 plays a role in the quality control check of RNA molecules. XRN2 co-transcriptionally degrades aberrant nuclear mRNA transcripts that result from defective 5’mRNA capping, splicing, or 3’end formation (6). XRN2 exonuclease rapidly degrades hypomodified tRNA and excess miRNA molecules, indicating that XRN2 likely regulates tRNA and miRNA quality control as well (7-9).
- Miki, T.S. and Großhans, H. (2013) Biochem Soc Trans 41, 825-30.
- Kilchert, C. and Vasiljeva, L. (2013) Biochem Soc Trans 41, 1666-72.
- Kim, M. et al. (2004) Nature 432, 517-22.
- West, S. et al. (2004) Nature 432, 522-5.
- Skourti-Stathaki, K. et al. (2011) Mol Cell 42, 794-805.
- Boisvert, F.M. et al. (2007) Nat Rev Mol Cell Biol 8, 574-85.
- Alexandrov, A. et al. (2006) Mol Cell 21, 87-96.
- Chernyakov, I. et al. (2008) Genes Dev 22, 1369-80.
- Großhans, H. and Chatterjee, S. (2011) Adv Exp Med Biol 700, 140-55.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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