WB, IP
H M R
Endogenous
50
Rabbit
#Q13426
7518
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro268 of human XRCC4 protein. Antibodies are purified by peptide affinity chromatography.
Background
DNA double-stranded breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells recognize and repair DSBs via two distinct but partly overlapping signaling pathways, non-homologous end joining (NHEJ) and homologous recombination (HR). DNA repair via the HR pathway is restricted to S and G2 phases of the cell cycle, while NHEJ can occur during any phase. NHEJ machinery is also utilized in V(D)J recombination, a process that generates diversity in immunoglobulin and T cell receptor genes. Defects in both pathways have been associated with human disease, including cancer (1).
DNA repair through the NHEJ pathway involves a core group of proteins that includes the Ku heterodimer (Ku70/Ku80), DNA-PKcs, DNA ligase IV, XRCC4, XLF, and PAXX (PAralog of XRCC4 and XLF, also known as C9orf142 or XLS). XRCC4 interacts with XLF and promotes the ligation of DNA strands by DNA ligase IV (2).
Mutations and polymorphisms in XRCC4 have been linked to human disease, including microcephaly, dwarfism, and cancer susceptibility (3-5). Knockdown of XRCC4 expression in hepatocellular carcinoma (HCC) cells and triple-negative breast cancer cells increases sensitivity to doxorubicin and ionizing radiation, respectively (6,7).
- Hartlerode, A.J. and Scully, R. (2009) Biochem J 423, 157-68.
- Tsai, C.J. et al. (2007) Proc Natl Acad Sci U S A 104, 7851-6.
- Murray, J.E. et al. (2015) Am J Hum Genet 96, 412-24.
- Bee, L. et al. (2015) EMBO Mol Med 7, 918-29.
- Saadat, M. and Saadat, S. (2015) J Med Biochem 34, 409-413.
- Lu, J. et al. (2017) Oncotarget 8, 87955-87970.
- Wen, Y. et al. (2019) Biosci Rep 39, BSR20180893. doi: 10.1042/BSR20180893.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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