WB, IP
H
Endogenous
21
Rabbit IgG
#Q9BQE4
55829
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys150 of human VIMP protein.
Background
VCP-interacting membrane protein (VIMP, selenoprotein S) is a putative reductase and endoplasmic reticulum (ER)-resident protein involved in the ER-associated degradation (ERAD) pathway (1,2). Research studies indicate that VIMP may play a protective role against inflammation and reduce ER-stress (3). The VIMP protein is a single-pass, transmembrane protein that recruits the cytosolic p97/VCP AAA-ATPase and its cofactors, UFD1 and NPL4, to the ER membrane (4). An ER membrane complex containing Derlin-1 and VIMP forms a critical node in the ERAD machinery and links substrate recognition in the ER lumen with the retrotranslocation function of the p97/VCP AAA-ATPase in the cytosol (1,4). Polymorphisms in the corresponding VIMP gene are associated with spontaneous preterm births and cardiovascular disease risk (5,6) while other studies do not support a correspondence between VIMP polymorphisms and inflammatory disorders (7).
- Lilley, B.N. and Ploegh, H.L. (2005) Proc Natl Acad Sci U S A 102, 14296-301.
- Christensen, L.C. et al. (2012) J Biol Chem 287, 26388-99.
- Fradejas, N. et al. (2011) Glia 59, 959-72.
- Ye, Y. et al. (2004) Nature 429, 841-7.
- Wang, Y. et al. (2013) PLoS One 8, e65657.
- Cox, A.J. et al. (2013) Acta Diabetol 50, 391-9.
- Martínez, A. et al. (2008) BMC Genomics 9, 329.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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