SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit (#76713) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

IHC-P

Product Information

Product Usage Information

1. To prepare the Vibrant Red working solution, to 5 ml SignalStain^® Vibrant Red Diluent add 2 drops SignalStain^® Vibrant Red Reagent 1 and 2 drops SignalStain^® Vibrant Red Reagent 2. Mix well before use.

2. After incubation with SignalStain^® Boost IHC Detection Reagent or other AP-based detection systems, wash slides and incubate with the working solution at room temperature for 20-30 minutes.

3. Wash slides in TBS-T for 5 minutes and then rinse slides in water. Counterstain, if desired.

Vibrant Red is compatible with permanent and aqueous mounting. Please note, permanent mounting requires dehydration through ethanol and clearing in xylene.

Optimal development times should be determined by the investigator. Working solutions should be used within 15 minutes of preparation. Do not heat Vibrant Red kit components or working solution.

The reaction product is also fluorescent and non-fading and can be viewed with TRITC filter systems.

Appropriate personal protective equipment should be worn when handling Vibrant Red. Dispose of in accordance with local regulations.

Storage

Substrate kit should be stored at 4ºC, protected from light. Each kit is stable for 12 months upon receipt if the reagents are not combined.

Product Description

The SignalStain^® Vibrant Red Alkaline Phosphatase Substrate Kit contains all of the necessary reagents to prepare a working solution of Vibrant Red for staining tissue sections. The Vibrant Red working solution reacts with alkaline phosphatase (AP) detection systems such as the SignalStain^® Boost IHC Detection Reagents (AP, Rabbit #18653, AP, Mouse #31926, AP, Rat #15764, and AP, Goat #26927), yielding a heat stable, red reaction product that is also fluorescent.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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SignalStain is a registered trademark of Cell Signaling Technology, Inc.

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