Revision 1

#5145Store at -20C

 

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Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
Vav1 (D45G3) Rabbit mAb 4657 40 µl 95 kDa Rabbit IgG
Vav2 (C64H2) Rabbit mAb 2848 40 µl 100 kDa Rabbit IgG
Vav3 Antibody 2398 40 µl 98 kDa Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Vav Isoform Antibody Sampler Kit is an economical way to examine the total protein levels of Vav isoforms 1, 2, and 3. The kit includes enough primary and secondary antibodies to perform four Western blot experiments.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Vav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho/Rac small GTPases. The three identified mammalian Vav proteins (Vav1, Vav2 and Vav3) differ in their expression. Vav1 is expressed only in hematopoietic cells and is involved in the formation of the immune synapse. Vav2 and Vav3 are more ubiquitously expressed. Vav proteins contain the Dbl homology domain, which confers GEF activity, as well as protein interaction domains that allow them to function in pathways regulating actin cytoskeleton organization (reviewed in 1). Phosphorylation stimulates the GEF activity of Vav protein towards Rho/Rac (2,3).

  1. Hornstein, I. et al. (2004) Cell. Signal. 16, 1-11.
  2. Crespo, P. et al. (1997) Nature 385, 169-172.
  3. Han, J. et al. (1997) Mol. Cell. Biol. 17, 1346-1353.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

    限制使用

    除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。

    专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品, (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

    Revision 1
    #5145

    Vav Isoform Antibody Sampler Kit

    Vav Isoform Antibody Sampler Kit: Image 1 Expand Image
    使用 Vav3 Antibody 对 Jurkat、MCF-7 和 HUVEC 细胞的提取物进行蛋白质印迹分析。
    Vav Isoform Antibody Sampler Kit: Image 2 Expand Image
    使用 Vav2 (C64H2) Rabbit mAb 对不同细胞类型的提取物进行蛋白质印迹分析。
    Vav Isoform Antibody Sampler Kit: Image 3 Expand Image
    使用 Vav1 (D45G3) Rabbit mAb 对 Jurkat、Raji 和 K-562 细胞的提取物进行蛋白质印迹分析。
    Vav Isoform Antibody Sampler Kit: Image 4 Expand Image
    一抗与靶标蛋白结合之后,与偶联 HRP 的二抗形成复合体。添加 LumiGLO®,在酶催化分解期间发光。
    Vav Isoform Antibody Sampler Kit: Image 5 Expand Image
    使用 Vav1 (D45G3) Rabbit mAb 对 Jurkat 细胞的 Vav1 进行免疫沉淀。使用同一抗体进行蛋白质进行印迹分析。