WB, IP, IHC-P
H M R Mk
Endogenous
65
Rabbit IgG
#Q53GS9
10713
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala367 of human USP39 protein.
Background
Ubiquitin specific protease 39 (USP39) is a 65 kDa protein that plays an important role in pre-mRNA splicing, as well as mitotic spindle formation. It displays significant homology with ubiquitin C-terminal hydrolase proteins (UCHs), containing both an N-terminal zinc finger domain as well as UCH-1 and UCH-2-like domains also observed in the UCH2 family of proteins (1). However, USP39 lacks a catalytic cysteine residue found in UCHs and has been shown experimentally to lack peptidase activity (2). USP39 associates with the U4/U6-U5 tri-small nuclear ribonucleoprotein (U4/U6-U5 tri-snRNP) complex and is necessary for the formation of the mature spliceosome. Silencing of USP39 has been shown to adversely affect chromosome segregation and cytokinesis in U2OS cells, likely due to improper splicing of Aurora B and other mRNAs necessary for mitotic spindle formation and checkpoint function (2). In addition, USP39 has been found to be overexpressed in many types of cancers, and in most cases is associated with tumor progression and poor prognosis. Overexpression has been observed in pancreatic (3), prostate (4), colorectal (5,6), lung (6,7), gastric (8), and triple negative breast cancers (9), as well as melanoma (10) and hepatocellular carcinoma (11,12).
- Makarova, O.V. et al. (2001) EMBO J 20, 2553-63.
- van Leuken, R.J. et al. (2008) Cell Cycle 7, 2710-9.
- Cai, J. et al. (2017) Biochem Biophys Res Commun 486, 184-90.
- Huang, Y. et al. (2016) Oncotarget 7, 22016-30.
- Yuan, X. et al. (2017) Oncol Rep 37, 2398-2404.
- Fraile, J.M. et al. (2017) J Biol Chem 292, 4164-75.
- Lin, Z. et al. (2016) Mol Cell Biochem 422, 97-107.
- Wang, X. et al. (2016) Mol Med Rep 14, 301-6.
- Liu, S. et al. (2015) Oncol Rep 33, 2477-83.
- Zhao, Y. et al. (2016) Tumour Biol 37, 13167-76.
- Yuan, X. et al. (2015) Oncol Rep 34, 823-32.
- Pan, Z. et al. (2015) Biol Res 48, 18.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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