WB, IP
H M R Mk
Endogenous
65
Rabbit
#P26368
11338
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu11 of human U2AF2 protein. Antibodies are purified by peptide affinity chromatography.
Background
U2 small nuclear RNA auxiliary factor 2 (U2AF2) is the large (65 kDa) subunit of the U2 auxiliary factor (U2AF) that plays an essential role in the splicing of pre-mRNA to generate functional mRNA transcripts. U2AF2 forms a heterodimer with the small (35 kDa) U2AF1 subunit to create the U2AF that recognizes the 3' splice site and facilitates spliceosome assembly (1-3). Research studies indicate that U2AF1 binds to the 3' splice site consensus AG dinucleotide at the intron-exon boundary while U2AF2 recognizes and binds the polypyrimidine tract upstream of the 3’ splice site. These two steps ensure accurate spliceosome assembly at splice sites (4-6). Mutations in U2AF2, which alter its splicing capabilities, have been described in various cancers (7,8).
- Jurica, M.S. and Moore, M.J. (2003) Mol Cell 12, 5-14.
- Kielkopf, C.L. et al. (2001) Cell 106, 595-605.
- Zamore, P.D. and Green, M.R. (1989) Proc Natl Acad Sci U S A 86, 9243-7.
- Merendino, L. et al. (1999) Nature 402, 838-41.
- Wu, S. et al. (1999) Nature 402, 832-5.
- Zamore, P.D. et al. (1992) Nature 355, 609-14.
- Glasser, E. et al. (2017) Biochemistry 56, 4757-4761.
- Maji, D. et al. (2020) J Biol Chem 295, 17148-17157.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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